Technology Of Microbial Pretreatment Of Straw Which Used For Photosynthetic Hydrogen Production

Biomass straw class has received attention,because its source is wide,price is low, and can be converted into energy through the microorganismfermentation,China’s abundant biomass resources, annual production of about morethan50, one hundred million tons (dry weight), about three times as much of China’scurrent primary energy consumption,It is of great significance for developingalternative energy and protecting the environment. Utilizing straw biomass tohydrogen production,because its hydrogen production conditions is mild,environment friendly and rich raw material sources has been believed to be the mainalternative form of hydrogen production in the future, the main problem of restrictingits use is the hydrolysis of lignocellulose in raw materials, researching and developingnew class straw biomass pretreatment technology become inevitable.This paper was financially supported by the National Natural Science Foundation(No.50976029)“Research on the ultrastructural biomass hydrogen producing processand metabolic hot”and National high technology research and development program(863program) project “the key technology research and demonstration of Biologicalhydrogen production”(No.2012AA051502).In this paper, using the reducing sugarcontent as the index to evaluate the cornstalk’s ability of hydrogenproduction,experimental study choosing Phanerochaete chrysosporium,Trichodermaviride, Aspergillus niger to pretreat the cornstalk of0.45mm and0.106mm with asingle species, two two joint, three combined.Optimizing out the best raw materialtype with straw biomass as a feedstock for hydrogen production parameters and thebest pretreatment class,aiming to provide a scientific reference for technology ofstraw biomass photosynthesis hydrogen production. Experiments show that.(1)After cornstalk was crushed respectively in0.45mm and0.106mm. Two kindsof particle size of cornstalk in the proportion of lignin, cellulose and hemicellulose aredifferent.In general, proportion of the lignin, cellulose and hemicellulose of0.45mmare higher than the particle size of0.106mm. (2)Phanerochaete chrysosporium,Trichoderma viride, Aspergillus niger can bevery good symbiosis between each other,and mixed culture of microorganisms growthspeed, coverage and extensions will be affected.(3)Through pretreating the0.45mm and0.106mm respectively with single strains,the sugar yard of0.106mm particle size of cornstalk is better than0.45mm size.theapperance time of maximum reducing sugar of the particle size of0.106mm cornstalkwhich was pretreated by Trichoderma viride and Aspergillus niger is earlier thanPhanerochaete chrysosporium.And the reducing sugar content of0.106mm sizewhich was pretreated by Aspergillus niger with18days was2.14mg/mL, effect oftreatment is the best.(4)Through pretreating the0.45mm and0.106mm respectively with two twojoint,sugar effect of0.45mm particle size of corn stalks was generally better than0.45mm(the0.45mm size cornstalk which was pretreated by Trichoderma viride andAspergillus niger was superior than0.106mm size),And at18th day,reducing sugaryard upped to the highest.0.106mm particle size of corn stalks which was pretreatedby Phanerochaete chrysosporium and Aspergillus niger had the best effect.Reachingthe maximum value4.23mg/mL in18days.(5)Through pretreating the0.45mm and0.106mm respectively with threecombined, sugar effect of0.106mm particle size of corn stalk was better than0.45mm size, the maximum of reducing sugar in0.106mm size of corn stover afterprocessing18days, reducing sugar content is:3.19mg/mL.