| Corydalis incisa is an excellent ornamental plant. Being the theoretical basis of artificial cultivation,researching seed dormancy characteristics, lifting methods, seed germination conditions is a priority.This study researched Corydalis incisa seed’s biological characteristics, seed dormancy, dormancy release methods, germination characteristics under the different hormone concentrations and temperature treatments. The results show that:Being embryo-after-seeds is the main reason for seed dormancy, not the bad seed coat permeability.Seed coat and embryo contained a certain amount germination inhibitors. The inhibition of seed coat was greater than seed embryo, methanol phase inhibition of seed coat was stronger than other separation phase, so the seed germination inhibition in material is one of the important reasons for the seed dormancy.Cold to warm temperature stratification seed’s germination rate was the highest in the three stratification, was the best way to stratification; Warm to cold temperature stratification seed’s embryo paraffin section and SEM scanning results showed that Corydalis incisa seed’s embryo have finished the physiological after ripening at the 120 d. Longer duration of stratification treatments, Corydalis incisa seed plasma membrane permeability, the content of soluble sugar and starch decreased gradually; the content of soluble protein showed "up-down-up" change trend; POD activity showed a trend of "down-up"; SOD total activity showed a trend of "down-up-down".Through cultivation of the appropriate temperature, and the use of hormones GA3 and 6-BA have effectively improved seed germination rate and germinating energy, promoted root growth, increased root ratio. The experimental results showed that the optimal treatment combinations of the seed germination rate was A2B2C1:GA3(100mg/l)+6-BA(5mg/l)+ temperature(5℃), the highest germination rate reached 65.33%; the optimal treatment combinations of the seed germinating energy was A4C1:GA3(500mg/l)+temperature(5℃), the 6-BA concentration had no significant effects on seed germination in this test. The optimal treatment combinations of the seed root length was A4B2C3:GA3(500mg/l)+6-BA(5mg/l)+ temperature(25℃); the optimal treatment combinations of the seed root ratio was A1B2C2: GA3(0mg/l)+ 6-BA(5mg/l)+ temperature(15℃). |