| High molecular weight glutenin subunits(HMW-GS) and gliadin fingerprinting of 105 barley cultivars(included 40 local cultivars, 65 improved cultivars) and 102 wild barley cultivars were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE) and acid polyacrylamide gel electrophoresis(A-PAGE), and the main results are described as follows:1. High molecular weight glutenin subunits(HMW-GS) compositions of 40 local cultivars were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE).The result showed that these cultivars only had one different band respectively, three kinds of HMW-GS were detceted, and they were named a,b and c subunit,of which 35 cultivars were a subunit, accounting for 87.5%; 3 cultivars were b subunit, taking up 7.5%; 2 cultivars were c subunit, accounting for 5.0%. This means that the HMW-GS of the local cultivars shows little variety and poor polymorphism.2.SDS-PAGE analysis of domestic improved cultivars was conducted, results showed that in 65 improved cultivars, 4 HMW-GS(a, c, d and e subunit) were found, resulting in 6 HMW-GS combinations, namely a, c, d, e, a+e and c+d. 34 cultivarswere a subunit, accounting for 52.30%, 3 cultivars of c subunit accounted for 4.62%, 21 cultivars of d subunit accounted for 32.3%, 4 cultivars of e subunit accounted for 6.15%, 1 cultivar of a+e subunit accounted for 1.54%, and 2 cultivars of c+d subunit accounted for 3.08%.3.SDS-PAGE analysis of foreign wild barleys was conducted, results showed that there were 19 kinds of subunits in the 102 wild barleys, which were 0.1*,0.15*,0.2*,0.3*,0.35*,0.4*,0.5*,0.6*,0.7*,1*,2*,4*,6*,7*,8*,9*,10*,12* and 20* subunit, resulting in 50 HMW-GS combinations.There were 14 single subunit, 25 two subunits, 12 three subunits and 1four subunits.4. A-PAGE analysis was used for studying the domestic barleys, and 99 different banding pattern were found. 23 kinds of band were separated. Judging from the frequency of every band in all materials, the variation range was between 5.94% and 77.23%. Great difference was shown in the three sections—α, β and γ. There were 6 variations in section α, 5 variations in β, 3 variations in γ and 9 variations in ω.The genetic similarity coefficient(GS) of the tested germplasm accessions varied from 0 to 1, and the mean value was 0.74. Cluster analysis indicated that the 102 accessions could be classified into 1 cluster at the level of GD 0.2, and into two clusters at the level of GD 0.235.5. A-PAGE analysis was used for studying the foreign wild barleys, and 102 different banding pattern were found as well as 36 different bands with various immigration rate. 2 to 20 gliadin bands were identified in each accession, with an average of 9.22. The significant difference was shown in the frequency of these bands. A new X section was found in section α. There were 5 band in section α, which had the highest frequency of one single bands. And there were 6 bands in section β, 14 bands in section γ and 14 bands in section ω. Additionally, 3 bands were found in the new section X.The results of genetic distance analysis showed that the mean GD was 0.82,ranging from 0.27 to 1. Most genetic distances of these cultivars were over 0.6, which indicated significant difference between these materials. Cluster analysis indicated that 102 accessions could be classified into 1 cluster at the level of GD 0.09, and into 6 clusters at the level of GD 0.18. The first cluster contained 72 accessions that could be further divided into two subcategories, of which the first subcategory contained 44 accessions and the second 35 accessions. The second cluster contains 12 accessions, the third 7 accessions, the fourth 4 accessions, the fifth 1 accessions, and the sixth 6 accessions. |
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