The Study On The Effects Of The Streptomyces Gongzhulingensis 769 Fermentation Production On Resistance Phytophthora Root Rot Of Soybean

In the soybean research, common model variety(Jack, Williams 82) and phytophthora megasperma variety with moderate susceptibility(Shennong No. 9) and high susceptibility(Jiunong No. 21) during production are selected, to research the influence of Streptomyces gongzhulingensis 769 on phytophthora megasperma during the growing process of soybean, and influence of Streptomyces gongzhulingensis 769 on total growth period and growth environment of soybean.The result shows that:(1) With the application of Streptomyces gongzhulingensis 769, the resistance to phytophthora megasperma of different soybean varieties have improvement of different degrees. Of which, the resistance enhancement of Williams 82 is the most significant, and the resistance of which is increased by 74.9 % compared with control group. The resistance enhancement of Jack is the lest significant, which is only 3.85%. The variety Jack with lest resistance change is selected to conduct research on influence of Streptomyces gongzhulingensis 769 on soybean disease induced resistance, and the result indicates that:When use Jack in pot incubation test, after watering with Streptomyces Gongzhulingensis 769 and inoculation, the PAL activity, PPO activity, POD activity, SOD activity, chlorophyll content, β-1,3-glucanase activity of soybean seedlings are much higher than that of controls. However, the CAT activity and MDA content are a little lower. It means that, when the soybean is infected with pathogenic bacteria, Streptomyces Gongzhulingensis 769 can improve the response speed and intensity of the soybean to pathogenic bacteria, thus improving disease resistance of the soybean.(2)The application of Streptomyces gongzhulingensis 769 can improve emergence rate of soybean. However, to different soybean varieties, the emergence rate increase is different. Of which, compared with control group, the emergence rate increase of Shennong NO. 9 is the most significant, which is 16.90 %, in addition:The height, length and width of leaf, stem diameter and length of axial root of soybeans watered with liquid of Streptomyces Gongzhulingensis 769 are over that of controls provided with clean water, which means Streptomyces Gongzhulingensis 769 helps the growth of soybean to some extent. Apply Streptomyces Gongzhulingensis 769 on Williams82 and Shennong 9, which are used in field experiment. The quantity and average weight of the bean pods harvested have increased: the quantity has increased by 31.53% and 5.60% respectively and the weight has increased by 10.49% and 11.72% respectively, while there is no big difference between the weights of 100 beans. Streptomyces Gongzhulingensis 769 can increase the soybean yield by contributing to the change of soybean branch and pod bearing, and it makes no difference for soybeans of different kinds.(3) Use Williams82 and Shennong 9 in field experiment. During their growth stages, it is found that the quantity of bacterial colonies in the soil watered with liquid of Streptomyces Gongzhulingensis 769 has increased significantly, while that of fungus colonies has decreased obviously. Besides, applying Streptomyces Gongzhulingensis 769 can improve the activity of biological enzymes in the soil such as urease, phosphatase, saccharase, catalase, etc. However, the improvement is different due to different soybeans, and after harvesting, the activity of urease, phosphatase, saccharase and catalase are higher than that before sowing, which means Streptomyces Gongzhulingensis 769 can inhibit the growth of fungus in the soil and improve soil fertility and microenvironment.