Differential Proteomic Analysis Of Blood Plasma And Milk In Early Pregnancy And Different Postpartum Period In Holstein Cows

Proteome analysis in plasma from Holstein cows in early pregnancy were performed using two-dimensional gel electrophoresis(2-DE) method combined with MADIL-TOF-TOF tandem mass spectrometry. Six proteins were up-regulated after breeding, which including TPA: conglutinin 1 precursor, transthyretin precursor, Chain A, Crystal Structure Of Bovine Serum Albumin In Complex With 3,5-Diiodosalicylic Acid(CSBSACDA), ALB protein, unnamed protein product and protein HP-25 homolog 1 precursor. The expression of conglutinin 1 precursor firstly increased and then decreased. The contents of transthyretin precursor and ALB protein fluctuated after estrus and breeding. The expression of Chain A, CSBSACDAincreased constantly. The contents of unnamed protein product and protein HP-25 homolog 1 precursor firstly increased and then kept stable. The contents of 6 proteins during pregnancy were higher than non-pregnant period. These proteins in the early pregnant plasma may be related to the immune response, thyroid hormone transport, prevention of amyloid fibril formation and transportation of iodine. Using the same methods to analyze the proteome in plasma from Holstein cows in the different postpartum period, 5 differential proteins were found. The expression of haptoglobin precursorand haptoglobin after delivery firstly increased and then decreased as the labor day. The expression of Ig gamma-2 chain C region(clone 32.2)-bovine(fragment) decreased at first then increased and maintained a steady trend at last. The levels of Chain A, CSBSACDA and Chain B, CSBSACDA after delivery were lower than on the 45 th day in the early pregnancy, and remained stable. These five proteins may be associated with postpartum stress, immune response and material transportation respectively. Proteome analysis in milk from Holstein cows in early pregnancy were performed using two-dimensional gel electrophoresis(2-DE) method combined with MADIL-TOF-TOF tandem mass spectrometry. Five differential proteins were identified. They are Chain A, CSBSACDA, immunoglobulin gamma 1 heavy chain constant region, Ig G1 heavy chain constant region, ALB protein and component PP3. The change of Chain A, CSBSACDA in milk firstly decreased then increased and decreased again. The contents of immunoglobulin gamma 1 heavy chain constant region and Ig G1 heavy chain constant region significantly decreased, then remained stable trend. The expression of component PP3 fluctuated, but it was higher during pregnancy than non-pregnant period. These proteins may be related to transportation, immune response and antibacterial. Using the same methods to analyze the proteome in milk from Holstein cows in the different period after delivery, eight differential proteins were found, which were serum albumin precursor, albumin, Ig heavy chain precursor(B/MT.4A.17.H5.A5)–bovine, alpha-s2-like casein precursor, beta-casein, kappa-casein precursor, c GMP phosphodiesterase alpha subunit and Keratin, type I cytoskeletal10. Alpha-s2-like casein precursor was only detected in the milk sample on the labor day. Beta-casein was detected in milk samples on the 7th day and the 35 th day after delivery, and the level on the 35 th day was higher than the 7th day. Kappa-casein precursor was highly expressed on the labor day, and then decreased rapidly and kept stable. Ig heavy chain precursor(B/MT.4A.17.H5.A5)–bovine existed in the milk samples on the 7, 15, 28 and 35 th day. The levels fluctuated, however, the level on the 35 th day was the lowest. The expression of serum albumin precursor from 7th day to 35 th day after delivery firstly increased and then decreased. The change of expression of albumin was contrary to the serum albumin precursor, and the level on the 35 th day was the lowest. The contents of c GMP phosphodiesterase alpha subunit from 7th day to 28 th day after delivery firstly increased and then decreased. The change of type I cytoskeletal10 from the 0th day to 28 th day after delivery remained stable. These proteins may be related to the passive immunization of colostrums, material transportation and lactation.