Study On Spore Propagation And Sporophytic Induction Technology Of Phyllitis Scolopendrium

The national level two endangered plants Phyllitis scolopendrium(L.)Newman. Which belongs to Phyllitis of Aspleniaceae as the research material is studied on conversion technology to fern sporophyte.This experiment studies the spore propagation and spore induction of the Phyllitis scolopendrium(L.)Newman. To the spore propagation and the spore body mainly to study the three parts: Firstly, to study the influence of spore germination of Phyllitis scolopendrium(L.)Newman spores with different sterilizing time processing, different pretreatment time, different storage time and different germination medium. Secondly, using the aseptic prothallus in the early culture as the research material were cultured on different inorganic salt concentration levels, different concentrations of sugar levels, different hormone types and concentration of medium, to explore the most appropriate induction conditions and proliferation conditions of prothallium under sterile condition. Thirdly, using the aseptic prothallus in the early culture as the research material to explore the the most appropriate induction conditions of sporophyte under usual condition including different substrate, different temperature conditions, different humidity levels and different illumination levels.The main results are as followings:1. The best germination effect is using 0.1% mercuric chloride for 4min,then the germination rate was 56.25%, the rate of pollution is relatively good for 70.66%, this result is better than that the pollution rate of 5min treatment is 83.33%,but the germination rate of 5min treatment is just 6.25%.2.Inoculability after using gibberellin to soak spore 10 min and 15 min gets the best germination effect, spore was germinated after 60 d,and the rate of germination arrives 95% and 86.67%%. But to using gibberellin to soak the spores had little effect on appearation of the cordate prothallus, cordate prothallus are generally appear after 10 d later germination.3. The Spore of P.scolopendrium(L.)Newman storage at 4℃ within 60 d in the refrigerator, each germination time and germination rate of treatment there was no significant difference.4. The most suitable medium of Phyllitis scolopendrium(L.)Newman spore germination is 1/4MS without sucrose; germination rate can reach 76.2%. Among them, different salt concentration has little effect on the germination rate, but to promote the role of inorganic salt concentration level is relatively low, sucrose had obvious effect on the germination time, do not add sucrose the spore germination time significantly ahead of schedule, it not only can be germination under 60 d.5. The germination time of spore of Phyllitis scolopendrium(L.) Newman affected by the moisture is maximum, when the water is adequate, the time which the germination of spore and the forming of prothallium in the river sand were the earliest, are just 42 d and 60 d respectively, but the germination rate is only 48.6%. The time which the germination of spore and the forming of prothallium in the peat were later than in the filter paper and in the river sand, but germination rate was the highest, reached 54.5%, and the spores from germination to the prothallus only needs 15 d.Therefore the most suitable for spore germination is peat matrix under routine conditions.6. The experimental results obtained the prothallus of Phyllitis scolopendrium(L.)Newman spore germination was the materials, the best proliferation medium is MS + 0.5mg/L6-BA + 15g/L sucrose, p H value is 5.8.Under this condition the proliferation coefficient was 7.61.7. The experimental results obtained the prothallus of Phyllitis scolopendrium(L.)Newman spore germination was the materials, the best induced medium is MS + 10mg/L GA3, and the induction rate reached 22.5%.8. Experimental result shows that the most suitable matrix induced sporophytes under conventional conditions is peat, the most transformation temperature of suitable environment is 25℃.When the relative air humidity reached 95%, the conversion rate can reach 87.6%. Furthermore, the conversion time is relatively shorter, just after 70 D can induce the first strain of sporophyte. And the light has little effect on the spore sporophyte.