Effects Of The Fermentation Concentrate Of Ganoderma Applanatum(Pers.) Pat On Main Performance And Immune Indexes Of Broilers

This experiment was conducted to study the effects of supplementation of the Fermentation concentrate of Ganoderma applanatum(Pers.) Pat,(GAC) on main performance and immune indexes of broilers. A total of 225 one-day-old healthy Arbor Acre(AA) broilers were randomly allotted to 5 treatments with 3 replicates of 15 broilers each. The five treatments were the control group(the basis diet), the antibiotic group(the basic diet with 5mg/kg flavomycin), the other 3 group with the polysaccharides content in the diet of 0.05%, 0.1%, 0.2%. The trial lasted for 42 days. The growth performance, immune organs indexes, immune organs morphology, lymphocyte proliferation, cytokine production, immunoglobulin production, Newcastle disease(ND) antibody and cytokine expression level in the spleen of broilers were determined to evaluate the immunological effects of GAC on broilers. 1. Effects of GAC on growth performance of broilersEach GAC group significantly increased the average daily gain and average daily feed intake compared with the control group and the antibiotic group(P<0.05). Each GAC group can decrease the feed conversion ratio and increase the survival rate. The results showed that: the dietary addition with GAC could improve the growth performance of broilers. 2. Effects of GAC on the immune organs of broilersFive broilers from each replicate were randomly selected and slaughtered to take thymus, spleen and bursa in order to study on the immune organs indexes, and immune organs morphology at the days of 21 and 42. Each GAC group significantly increased the thymus and spleen indexes compared with control group and the antibiotic group(P<0.05). Each GAC group co increase the bursa indexes, and had no significant effects on the various organs and tissues form. The results showed that: the dietary addition with GAC could increase the immune organs indexes and had no significant effects on the various organs and tissues form. 3. Effects of GAC on lymphocyte proliferation of broilersAt the day of 14, 21, 28, 35 and 42, MTT method to determine the T/B lymphocyte proliferation of broilers. 0.05% GAC group significantly increased T lymphocyte proliferation of broilers. The experiment was designed to study the effects of GAC supplementation on the(P<0.05); 0.1% GAC group significantly increased B lymphocyte proliferation of broilers(P<0.05). The results showed that: the dietary addition with GAC could increase the lymphocyte proliferation of broilers. 4. Effects of GAC on cytokine and immunoglobulin production of broilersAt the day of 14, 21, 28, 35 and 42, ELISA method was used to detetmine the cytokine and immunoglobulin production of broilers. 0.05% GAC group significantly increased cytokine production of broilers(P < 0.05); 0.1% GAC group significantly increased immunoglobulin production of broilers(P<0.05). The results showed that: the dietary addition with GAC could increase the cytokine and immunoglobulin production of broilers. 5. Effects of GAC on Newcastle disease(ND) antibody of broilersAt the day of 14, 21, 28, 35 and 42, Hemagglutinauon inhibition(HI) experiment to determine the ND antibody of broilers. The results showed that: the dietary addition with GAC could increase the ND antibody level of broilers. 6. Effects of GAC on cytokine expression level in the spleen of broilersFive broilers from each replicate were randomly selected and slaughtered to take spleen at the days of 21 and 42. RT-PCR method was used to determine the cytokine exdpression level in the spleen of broilers. 0.05% GAC group significantly increased the 3 cytokine expression level in the spleen of broilers compared with the control group and the antibiotic group(P<0.05). The results showed that: the dietary addition with GAC could increase the cytokine exdpression level in the spleen of broilers.