Effect Of Submerged Fermentation Concentrate Of Hericium Caput-medusae(bull.:Fr.)Pers. On The Cecum’ Lactobacillus In Broilers

This experiment was conducted to study the effect of submerged fermentation concentrate ofHericium caput-medusae (Bull.:Fr.)Pers. on the cecum’ Lactobacillus in broilers. A total of300one-day-old AA broilers were divided randomly into5groups with3replicates of20broilerseach. The control group was fed by the based diets, the antibiotic group was fed by the baseddiets with0.5%flavomycin,the experimental groups were fed by different amount of HFC in thebased diets(concentrations of polysaccharide in the based diets was0.1%and0.3%and0.5%)during the60days study.The results as followed:1. In the HFC,the polysaccharide was18%, the water was33.08%, CP was4.68%, the adiposewas11.29%, the fiber was0.39%, the ash was1.99%, the energy was4.13MJ.Kg-1, thecontent of Ca was3.86%, the content of P was1.95%.2. The results of the production performance with broilers：in21days, the group of HFC couldincrease the average daily gain and reduce the feed conversion rat；in42days,the group ofHFC could increase the average daily gain and the average day intake，at the same timereducing the feed conversion rat；in60days, the0.1%group of HFC could increase theaverage daily gain and reduce the feed conversion rat. In a word，HFC could promote theproduction performance of broilers.3. The quantitative analysis method of real-time FQ-PCR on the Lactobacillus was established,the regressing equation Y=-3.337*LOG(X)+35.31, the relative coefficient R2=0.998, theamplification efficiency was99.4%. The sensitivity：template detection limit was47.3copies/μL; the specificity: the melting curve was single, indicating that the method wasspecific; the repeatability: the intra-assay coefficient of variation was0.31%and0.3%, theinter-assay coefficient of variation was1.21%and1.45%in the standard of4.73×107and4.73×105.4. The quantitative analysis method of real-time FQ-PCR on the Lactobacillus was established,the results showed that: in21days, the0.1%group of HFC could significantly increase theamount of Lactobacillus（P<0.05）; in42days, the0.1%0.3%0.5%group of HFC couldsignificantly increase the amount of Lactobacillus（P<0.01）; in60days, the0.5%group ofHFC could significantly increase the amount of Lactobacillus（P<0.05）. In a word, HFCcould increase the amount of Lactobacillus.Conclusion: 1.The quantitative analysis method of real-time FQ-PCR on the Lactobacillus was established.2. HFC could promote the production performance of broilers, and the optimum supplemental levelis0.1%.3. HFC could increase the amount of Lactobacillus in the cecum of broilers, and the optimumsupplemental level is0.1%.