| This paper focus on the technique and theory applied in macroalgae transplantation and growth development and had a primary study on application of macroalgae in the construction of artificial alga reefs based on reproductive biology. Experiment of macroalgae transplantation and cement pipe substratum of Sargassum thunbergii artificial breeding completed during April to October in 2014, liquid-phase oxygen electrode system, chlorophyll-fluorescence techniques and laboratory culture method were used to determine the effects of illuminance, temperature, nutrient level on photosynthetic characteristics, antioxidant system and chlorophyll fluorescence parameters of Laminaria digitata, Macrocystis pyrifera, Sacchrina japnocia. The results showed that:(1) The experiment transplantation of Hizikia fusifarme, Sargassum thunbergi, Zostera marina was conducted near national aquatic germplasm nature reserve at early May in 2014. There were 1.521 million young sporophytes of Sargassum thunbergi and 3.231 million young sporophytes of Hizikia fusifarme after 45 daystransplantation. Incidence of new branches of S. thunbergi is 3~6 per plant, average length is 6.5 cm. Average length of Z. marina is 22.7 cm and average density is 9.3 individual in each meter.(2) The experiment of cement pipe substratum of S. thunbergii artificial breeding conducted at Laizhou during June to August in 2014, there were 1.8 million young sporophytes of S. thunbergi after 60 days-cultured and average length of sporophyte is 0.97 cm. Average density is 8.7 individual in each square centimeter.(3) The aim of this study was to observe what effect temperature stress(at increments of 3, 8, 13, 18 oC) has on the antioxidant system of Laminaria digitata. The variables that were monitored included, growth, soluble protein content, maleic dialdehyde(MDA) content, antioxidant enzymes and chlorophyll fluorescence parameters. The results can be summarized as follows: 1) the highest values for relative growth rate(RGR) was observed at 13 oC, and growth rate was significantl different to that of 3, 8 and 18 oC(P<0.05). 2) The soluble protein content of samples at 18 oC was significant higher(P<0.05) than group 13 oC, and values were highest when stressed at the different temperatures for 4h. After 24 h of temperature exposure, soluble proteins content of samples at 13 o C increased to 16.4% and reached the highest values. 3) MDA content firstly increased within 4h then decreased within the stressed time of 48 h. After 4h, group 3 and 18 oC had higher MDA contents than groups 8 and 18 o C, and the highest value for all samples was observed at 18 oC, which was significantly different to the other groups(P<0.05). 4) After 36 h treatment, CAT activity of groups 13 and 18 oC firstly increased then decreased. At 24 h, group 13 o C reached the highest CAT values that were significantly different to other temperature groups(P<0.05), SOD activity of group 3 and 8 oC firstly decreased within 4h then increased and reached the highest values after 24 h. 5) The Fv/Fm, r ETRmax of samples at 3 oC treatment were significant lower than at groups at 13 oC, the value of Fv/Fm decreased with stressed time and r ETRmax reached the highest values after 4h of temperature exposure at 18 oC, r ETRmax of samples at 8 oC were significant higher than the other groups after 36 h treatment.(4) For clarifying the development and ecological adaptability of juvenile sporophytes of Macrocystis pyrifera, the effects of photoperiod(10 L: 14 D, 12 L: 12 D, 14 L: 10 D, 16 L: 8 D) on gametophyte clone seed-rearing of Macrocystis pyrifera were studied. And, the impact of light intensity, temperature, nutrients concentration on the growth, net photosynthetic rate(Pn), dark respiratory rate(Rd) of sporophytes(0.5~1 cm) of Macrocystis pyrifera were investigated by single factor test and orthogonal experiment. The major results included: 1) Large numbers of gametophytes developed into sporophytes at long photoperiod(14 L: 10 D, 16 L: 8 D). 2) The highest values for relative growth rate(RGR) and Pn were observed at 70 μmol/(m2·s) light intensity, 13 oC temperature and 2 mg/L nitrogen concentration(N/P=10:1, the same below). The growth of sporophytes were inhabited when exposure to 90 μmol/(m2·s), RGR of which significantly lower than other tests. RGR at 10 oC was significantly lower than at 13 oC(P<0.05). And RGR significantly decrease when exposure to 8mg/L nitrogen concentration. 3) Pn and Rd present the rising trend with increased of nutrients concentration. Pn of 0.2 mg/L group significantly lower than 6 mg/L and 8 mg/L group. Rd significantly difference compared with other groups while cultured at 0.2 mg/L(P<0.05). 4) Pn decreased with rising of temperature while cultured at the same light intensity. At the range of 40~80 μmol/(m2·s), Pn was significantly difference between 10 oC, 13 oC group and 16 oC, 19 oC group.(5) The aim of this study was to observe what effect of high temperature stress(25 oC) on the antioxidant system, pigment content and chlorophyll fluorescence parameter of. The major results included: 1) soluble protein content of 25 oC group reached the maximum value after stressed for 12h(P < 0.05), a 37.9% increase compared to the initial conditions. 2) MDA content of 25 oC group increased with stress time and reached highest value after stressed for 48 h, which compared with the control group significant difference. 3) Pigment content of 25 oC group decreased with prolong of stress time. It reached the minimum value after stressed for 48 h(P < 0.05), a 37.9% decline compared to the initial conditions. 4) Under the condition of high temperature, POD activity decreased within the stressed time and reached the minimum value after stressed for 48 h(P < 0.05), a 38.5% decline compared to the initial conditions. SOD activity showed a trend of fluctuations and reached the minimum value after stressed for 8 h. After stressed for 40 h, SOD activity reached maximum value and 36% increase compared to the initial conditions(P < 0.05). CAT activity decreased within the stressed time and reached minimum value after stressed for 48 h(P < 0.05), a 75.8% decline compared to the initial conditions. 5) The value of Fv/Fm of 25 oC group decreased with stressed time and reached the minimum value after stressed for 48 h(P < 0.05), a 56.8% decline compared to the initial conditions. |
PDF Full Text Request