| With the development of the high-yield, high-quality and efficient agriculture, and the requirement for green agriculture and food, the awareness of the importment for soil improvement and scientific fertilization were enhanced, thus promoting the research and exploration of the new technologies for producing fertilizers. Plant growth-promting rhizobacteria an important group of soil microorganism have received increasing attention in different research fields such as soil microbiology, plant pathology and so on, and it also become a research hotspot of ecological agriculture. Many PGPR strains and their products have been used in agriculture, forestry, environmental remediation and so on.In this study, the PGPR strain Bacillus amyloliquefaciens SQR9 which have growth-promoting and biocontrol function was used to develop bio-nursery substrate and bio-organic fertilizer and the effects of these two products were further studied for providing the theoretical and technical support for exploring the nover PGPR products.The seeding experiment showed that bio-soilless media produced by keep-alive addition of different contents of the function strain SQR9 in the nursery substrate could promote the growth of cucumber and eggplant, and the strain SQR9 could colonize in the two plant rhizospheres. More over, compared to the eggplant, the colonization ability of strain SQR9 in the cucumber rhizosphere was significantly higher. For pot experiment, compared to the eggplant, after transplanting, the growth promoting effect for cucumber was more obvious, and the number of functional bacteria in the rhizosphere of cucumber was significantly higher. Cucumber fusarium wilt in pots transplanted with seedings grew in bio-soilless media with 10%(v/w) addition of strain SQR9 was significantly suppressed and the disease control rate is 40.39%.This study investigated the possibility to produce bio-organic fertilizer using algal sludge as a solid-state fermentation (SSF) medium. Results showed that addition of algal sludge contributed to efficient SFF by a plant growth-promoting rhizobacteria (PGPR) strain SQR9. At the end of compost, the treatment of cattle manure compost mixed with algal the fertilizer germination index up to 96.00%, and the total microcystin degradation rate of the processing of N-BIO up to 77.34%. The rate of nitrogen loss of cattle manure compost mixer with algal treatment was 18.02%, Finally, the cattle manure compost was selected as the organic fertilizer mixed with algal slddge. The optimum algal sludge content was 5%(DW, the same below), and the initial inoculation size was 5% also. Plant growth experiments showed that the produced algal bio-organic fertilizer efficiently promoted plant growth.By adding rapeseed cake and two high-protein solid wastes, algal sludge and feather powder, in cow dung compost through single-factor test and orthogonal experiment to produce bio-organic fertilizer with PRPG strain Bacillus amyloliquefaciens SQR9. Single-factor test results showed that the optimum inoculum volume and moisture content were 7.5% and 40%, respectively. Orthogonal test revealed that the cell density of strain SQR9 could reach maximum in the optimum combination consisting of 5% algal sludge, 8% rapeseed and 12% feather powder. This combination was chosen for the expanded SSF process, the number of strain SQR9 approached 7.78×108 CFU/g DW. The pot experiment results indicated that the bio-organic fertilizer application efficiently promoted the eggplant and cucumber growth.Three bio-organic fertilizers produced by traditional method of our laber (BOF), by cow dung compost and algal sludge (BIO Ⅰ), and by orthogonal optimization method (BIO Ⅱ) were chosen for the eggplant field experiment. Results showed that bio-organic fertilizer could increase the yield and fertilizer agronomic use efficiency. The bio-organic fertilizer BIO Ⅱ reached the highest yield (2750.50 kg/acre) followed by BIO I (2694.39 kg/acre), and the lowest yield was observed in BOF treatment and the value was 2516.73 kg/acre. In addition, Bio-organic fertilizer application could increase the population of soil microbes. |
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