| Mite disease is a parasitic disease which is caused by Sarcoptes scabiei or Psoroptes cuniculi.Now its one of the main disease in animal husbandry industry,but the current commonly used miticides are mainly chemical acaricides which has environment safety problems such as high toxicity, environmental residue. the 9-oxo-10,11-dehydroageraphorone (euptox A) is a cadenine sesquiterpene from Eupatorium adenophorum against S.scabiei and P.cuniculi was tested in vitro. The aim of this study is to extracted euptox A from Eupatorium adenophorum and to explore Clinical acaricidal effect against P.cuniculi in rabbits and the preliminary evaluation with acute experiment.Not only looking for new drugs for mite disease,but also for provide a scientific basis for the development of Eupatorium adenophorum. The main contents and results were as follows:1.Clinical efficacy of euptox A extracted from Eupatorium adenophorum against P. cuniculi in rabbitsThe thirty rabbits were divided into five equal groups at random (groups A, B, C, D and E). Groups A, B and C were treated at days 0 and 7 with 4,2 and 1 mg/ml of euptox A, respectively. Groups D and E as the positive and untreated control groups, respectively. In the treatment process, each of the left and right ears were treated with the use of a cotton swab dipped with 2 ml of the euptox A, then embrocated treatment as an ointment. The presence of clinical signs was assessed daily and any abnormal reactions were recorded. Detailed examinations were performed on days 0,7 and 14 which included an assessment of the presence or absence of mites and an evaluation of clinical infection and the degree of recovery. The results show:All infected rabbits treated with 4,2 and 1 mg/ml of euptox A (groups A, B and C) recovered and were completely cured by day 14. Athough redness and inflammation were found in parts of the ear canals. However, after the second treatment, the infected rabbits in group A-C were cured completely. Rabbits in group D (positive control) exhibited improvements in clinical signs during the experiment and no inflammation was observed. However, rabbits treated with negative control showed only minor signs of aggravated.2.The initial safety test on euptox AThe thirty mouses were divided into five equal groups at random (groups A, B, C, D and E) in acute oral test. Groups A-E were irrigated with 1g/kg、1.5g/k、2.3g/kg、 3.4g/kg and 5.1g/kg of euptox A once.Then To observe the death situation of mouse, dissect the dead mouses, observe the pathologic changes of the relevant organ.To kill all the mouse at day 8. The results show:a mouse dead in group C,three mouse dead in group D, five mouse dead in group E. After calculation, the LDso of euptox A was 3.45g/kg by means of oral, its 95% confidence interval was between 2.58g/kg to 5.04g/kg and toxicity regression equation was y=-3.28+6.11x(R2=0.987). In a word, euptox A are low-toxic drugs.The six rabbits were used to as experimental animal by means of skin coating.Euptox A was daubed on the left boby,the right boby as untreated control groups were daubed normal saline. he results show:there were no changes for rabbits in 72h. Erythema and edema was not seen in the experiment part, skin evaluation of rabbits was 0. In a word, euptox A are nontoxic drugs. |
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