| The resources needed for plant growth and reproduction, such as light, water, soil nutrients, are usually distributed in space. Clonal plants can through physiological integration to improve or relieve local heterogeneity in the pressure to promote the ramet growth and habitat heterogeneity of colonization. The integration of carbon and nitrogen is a very important part in the physiological integration of clonal plants, which is the material basis for the survival, growth and reproduction of clonal plants. Clonal plants on soil nitrogen uptake sites diversification and intake of rear transmission pattern and process complexity is in general non clonal plants do not have. Especially when the habitat patch of clonal plants showed heterogeneity, the distribution and pattern of the transmission and distribution of nitrogen were more complex.In this study, by combining suffered heterogeneity of light treatment of progeny lines underground N supply effectiveness and root rhizosphere soil microbial community diversity of changes, to discuss if the lighted offspring rametstransfer photosynthate to shaded offspring ramets and to discuss if the photosynthate affected the soil nitrogen availability by root exudates. The research is important for reveal the clonal plants in heterogeneous suffered light according to the conditions of growth mechanism. After Phyllostachys nigra ramets were treated in connection and severed state respectively for three months under heterogenous light conditions, C, N related indicators of rhizosphere soil around each ramet, including organic carbon(SOC), total nitrogen(TN), dissolved organic carbon(DOC), dissolved organic nitrogen(DON), ammonia nitrogen(NH4+-N), nitrate nitrogen(NO3-- N) were measured, and using phospholipid fatty acid method for qualitative and quantitative analysis of each ramet rhizosphere soil microbial communities to determinate concentration and distribution ratio of each bacteria species; by using principal component analysis, correlation and significant analysis, combined with the domestic and foreign related research, it is concluded that the main conclusions are as follows:(1) SOC, TN, DOC, NH4+-N concentrations of shaded Phyllostachys nigraoffspring ramets showed a significant difference between connected and severed treatment. and SOC, TN, DOC, NH4+-Nconcentration of connected shade offspring ramets were higher than the severed shade offspring ramets identified that clonal integration improves soil carbon and nitrogen utilization effectiveness for connected shaded offspring ramets.(2) PLFAs concentration of rhizosphere soil microbial for Phyllostachys nigra showed a significant difference(P <0.05) between the two treatments of shading connected and severed conditions,; Actinomycetes, fungi and Gram negative bacteria PLFAs ratio under the state of connected is significantly increased 67.31%, 17.59%, 11.75%, respectively, compared with those of severed shade state. and general bacteria decreased relatively. It showed that clonal integration affected the concentration and distribution of the strains in the rhizosphere soil.(3) Principal component analysis showed that actinomycetes, fungi, bacteria in the rhizosphere bacteria between different treatments for Phyllostachys nigra have been included in the original sample data in 100% of the information, the three indicators can be used to explain each bacterium changes in root rhizosphere soil microbial community under the different treatment(4) Phyllostachys nigra can wisely reduce the percent of bacteria in soil which nitrogen utilization effectiveness is low, and increase the percent of actinomycetes and fungi by clonal integration. in order to improve the soil nitrogen utilization efficiency of whole Phyllostachys nigra. |
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