Taxonomy Of Some Chinese Cordyceps-colonizing Fungi And Evalution Of Their Secondary Metabolites

Chinese cordyceps is valubale traditional Chinese medicine and endemic in the Qinghai-tibet plateau. As entity of caterpillar and fungus, manys fungi colonize on natural Chinese cordyceps.During the long term evolution, those fungi have the close relationship with Ophiocordyceps sinensis in similar secondary metabolites with pharmacological activities, and were developed into the mycelium products.Therefore,we make a hypothesis that the medical function of natural Chinese cordyceps may coordinate between the Ophio-cordyceps sinensis and colonized fungi. The fungi associated with the natural Chinese cordyceps are important fungal resources.During survey of fungi associated with natural Chinese cordyceps, a number of fungi were isolated and preliminarily identified. We selected 28isolates that could be new taxa for further study in details. After morphological observation and ITS sequence analysis, eight strains have lowere homology with known fungal species. However, only three strains canproduce spores.Based on the morphological observation and mult-genes analysis,two strains are represented two new species have been proposed and described.We also selected some dominant strains based on geographic pattern to investigate their antimicrobial and antioxidant activities.The crude secondary metabolite extracts of the selected fungi were prepared after culturing on rice substrates for thirty days. The antimicrobial activity showed that strains of SC13-35F-1、SC13-29F-1、QH13-1F-1 and 19A to Fusarium nivale have antimicrobial activity; The antioxidant activity assay showed that strain of the SC13-35F-1、YN13-6F-1、SC13-52F-1、6667、6659、6670 and 18A have obvious antioxidant activities among the crude extracts of 15 strains of the Chinese cordyceps-colonizing fungi.The fungi with antimicrobial and antioxidant activity have potential for further investigation to develop pharmaceutical drugs and healthty products.