Partical Characteritics Concerning Biology And Ecology, And Antidepressant Effect Of Ophiocordyceps Sinensis

Ophiocordyceps sinensis is the most famous and precious Chinese medicine. The wild resource of O. sinensis is extremely scarce due to over-harvest. Identification of the anamorph of O. sinensis is very important for development products using its mycelia and protection of the wild resource. In this stuy, Hirsutella sinensis was identified as the asexual of O. sinensis by microcyclic conidiation and molecular phylogenetic analysis. Antidepressant effect of the H. sinensis was confirmed. Additionally, the diversity of O. sinensis symbiotic bacteria was analyzed. The main results obtained in this study were concluded as the follow.1. Partical biological characteristics of O. sinensis were characterized by measured 30 fruits collected from Deqin of Yunnan. Results indicated that the growth rate was 0.12-0.74 mm/day under artificial control condition. There was a positive relationship between the ascospore biomass and stroma diameter (r=0.95).2. The process of microcyclic conidiation concerning O. sinensis was illustrated by method of slide culture. Ascospores germinated after inoculation of 20-35 days, which began to produce conidiophore and conidia. The morphological characteristics of conidiophore and conidia were described. By microcyclic conidiation, H. sinensis were confirmed as the asexual of O. sinensis.3. By phylogenetic analysis of the ITS sequences of stroma, caterpillar and ascospore, and their asexual strains originated from 3 freuits, H. sinensis was confirmed as the asexual of O. sinensis.4. The antidepressant effect of O. sinensis mycelia was confirmed based on the results of tail suspension test and forced swimming test when feeded the mouse with the dose of 5 g/kgoday.5. The diversity of O. sinensis symbiotic bacteria and the bacteria in surrounding soil was compared. Results showed that the diversity of O. sinensis symbiotic bacteria (diversity index=3.26) was lower than soil bacteria (diversity index=4.16).6. By method of polytaxonomic taxonomy, OBM34, a strain of O. sinenis symbiotic bacteria, was identified as a new species of the genus Sphingobacterium.