Summer Squash PRSV、CMV Resistant Genetic Analysis And Preliminary Resistance Gene Positioning

Papaya ringspot virus watermelon strain(PRSV-w) and cucumber mosaic virus(CMV) is the process of planting summer squash squash production of major hazards virus damage. In susceptible varieties were homozygous inbred Hubafu(P1A) and disease-resistant varieties of 08-1(P2A), Qianyibai(P1B) and disease-resistant varieties of 08-13(P2B) Construction of two 6 generations genetic groups A and B, to summer squash PRSV-w, CMV inheritance of resistance was studied. Using 732 pairs of SSR primers and 38 pairs of EST-SSR primer pairs summer squash PRSV-w, CMV resistance locus initial positioning.Research indicates:1 Summer squash anti PRSV-w resistance trait is a trait controlled by nuclear genes, PRSV-w susceptible to disease manifestations of incomplete dominance, is the quality trait controlled by a single gene. summer squash anti-CMV resistance trait is a trait controlled by nuclear genes, CMV susceptible to the disease is not completely dominant performance, quality traits controlled by a single gene.2 Design [L9(3) 4] orthogonal experiment, get the best summer squash SSR-PCR system: 15 uL system Mg2 + was 1.5mmol / L, Taq enzyme 2.2U, primer 0.3umol / L, dNTPs0.3mmol / L, 10 × Buffer, 50 ng template DNA.3 Summer squash anti PRSV-w of SSR Molecular Markers: with 732 pairs of SSR primers were 38 pairs of EST-SSR primers for PRSV-w A group of parents present and F2, B groups and their F2 resistant and susceptible pools will be the A group and CMV parents and F2, B groups and their F2 resistant and susceptible pools were amplified, which PRSV-w the a group has m162, m140, m29, m112, mc43, mc64 and m80 seven sets of primers to amplify the pool of anti-flu having polymorphic bands, including B group have m141, m162, m126, m64, p265, p201 and m80 seven groups in the anti-sense primer pool amplified polymorphic bands. This 7 Group primers F2 generation plant validation and linkage analysis showed that screened labeled primers m80 and m162 zucchini anti PRSV-w resistance locus linkage, genetic distance 3.7cM, specific fragment obtained approximately 200 bp. CMV failed to get a primer in this batch chain primers.