Construction Of N Linkage Genetic Map Of Soybean And Bioinformatics Analysis Of CLC Homologous Gene Family In Soybean

According to the information of public soybean genetic map provided by SoyBase, chosing 22 SSR markers from the N linkage group of soybean, and using Nannong 1138-2, 3060 and its hybrids （G combination, F2 generation, a total of 110 plants） as the experimental materials, we selected 10 polymorphic SSR markers to build N linkage group genetic map after screening polymorphism. Result showed that in addition to the 2 SSR markers （Satt257 and Sat₃79） was outside the group, and 8 SSR markers could be linked to the group. But the group was divided into two parts; compared with N linkage group of the public map, there was a larger distance between markers, and the order of Satt530, Satt237 also displayed differently.By using NCBI, Phytozome database and relevant bioinformatics software, the systematic prediction, analysis and comparison of the homologous CLC family in soybean genome were conducted. The results showed that the soybean CLC homologous genes were located on No. lth,5th,9th,11th,12th,16th and 19th chromosome,the physicochemical properties of CLC proteins are generally consistent, only Glyma01g44950 and Glymallg00690 protein are unstable proteins and other proteins show is stable; amino acid sequence alignment analysis found that CLC have（I）GS/PGIPE, （II）GKE/AGPLVH and （III）PVGGVLFALEE/G three amino acid residues conservative area; evolutionary selection pressure and functional divergence analysis shows that soybean CLC homologous genes may be experiencing purifying selection, and there is functional differentiation among the multiple soybean CLC copies during evolution.The seedlings of wild soybean accession BB52, cultivated soybean cv. N23674 and their hybrid strain 4076 were treated with 150 mmol·L-1 NaCl solution for 24 h. The expressions of four soybean CLC homologous genes （Glyma09g28620, Glymallg00690, Glyma13g23080 and Glyma19g25680） were analyzed by semi-quantitative RT-PCR, the results showed that during the process of 24 h salt stress, different transcriptional patterns of the 4 genes were displayed in the roots and leaves of BB52, N23674 and 4076 seedlings, and the expression of Glymal9g25680 was more significant than others whether in the response rate, time duration or expression abundence.