| Zhedong white goose is an improved variety among the Chinese goose. But its egg production is low because of its broodiness. That’s what hindered the development of this variety. By observing the ovary of female goose in broody period, we found atresia follicles occurred in different stages. Numerous studies show that the apoptosis of granulosa cells is the key factor of follicular atresia. In mammals, studies show that apoptosis is closely related to autophagy. Therefore, we attempt to study the perspective mechanism of follicular atresia by autophagy.The autophagosome was targeted by Transmission Electron Microscope and Immunohistochemistry in goose follicles. In order to determine whether a follicular atresia is associated with autophagy, we used Western blot and qPCR to test the expression level of LC3(Microtubule?associated protein light chain 3). At the same time, to define whether the autophagy inducted by oxidative stress is the factor of follicular atresia, we determined multiple biochemical indexes. To validate the result above, we established an oxidation stress model of granulosa cells at the cellular level. Then, we used the rapamycin and 3-methyladenine to test the autophagy signaling pathway.This experiment indicated the autophagy level of granulosa cell in goose atresic follicle rose(P<0.05), while the oxidative stress level increased(P<0.05). We established an oxidation stress model of granulosa cells by H2O2, the results showed that the autophagy level increased(P<0.05). When added 3-MA or rapamycin to coprocessing, the expression of LC3-Ⅱ was increased observably(P<0.05) by rapamycin, the number of autophagosomes notably rose(P<0.05), otherwise, 3-MA had no effect on the autophagy level of follicular granulosa cell.The results indicate that follicular granulosa cell autophagy is related to follicular atresia in goose. Oxidative stress is an important induced factor of granular cell autophagy, and it is regulated by the mTOR signaling pathways. |
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