| This study to our laboratory screening for aflatoxin B1 stronger adsorption Lactobacillus Plantarum making as the research object, study of the adsorption effect, both inside and outside the feed China. simple factor design of experiment,39 kg chicken feed will be randomly divided into 13 processing group, each set three repeat treatment group, each group 1 kg of feed. Group A for control group, the other 12 to 4 groups respectively for the unit to join the 10,20,40μg/kg of AFB1, and then respectively in each of the four groups of the number of living bacterium content is 0, 106,107,108 CFU/g Lactobacillus Plantarum, at 0,15,30 d, respectively in each group by 50g feed samples, with feed of AFB1l ELISA kits contain the amount of aflatoxin B1. One hundred and twenty 1-day-old healthy Avian broilers were randomly divided into three groups, each four replicates of 10 broilers. The three dietary treatments consisted of Control Group (conventional feed as a negative control diet), AFB1 Group (50μg/kg aflatoxin B1), L. Plantarum Group (50μg/kg aflatoxin B1 plus 0.1% Lactobacillus Plantarum F22). Test diets were offered for 28 days continuously and the birds were sacrificed. During the test, with 14,28 days of age, as a point in time determination of growth performance,28 d, the fecal samples collected for the determination of AFB1 content. Feeding trials after each processing random 8 chickens, conventional creations take heart, liver and muscle tissue, to observe the Hepatic Sample Microscopic Analyses, determination of heart liver organs index, The aflatoxin B1 residues in the liver, muscle and faeces. The results as show:1. AFB1 in the feed:feed plants of Lactobacillus Plantarum in the treatment group was decreased significantly compared with aflatoxin group (P<0.05), the each other significant difference between the treatment group (P<0.05), and as to join Lactobacillus Plantarum number of living bacterium, an increase in the number of AFB1 content in the feed quantity is gradually reduced. the content of AFB1 show a rising trend During the whole storage.2. Growth performance:14 days of age, AFB1, L.Plantarum average weight (AW), average daily gain (ADG) and average daily feed intake (ADFI) was significantly lower than control group (P<0.05), feed conversion ratio (F/G) was significantly higher than that of control group (P<0.05), the AFB1 group, no significant difference between the L.Plantarum group (P>0.05); 28 days of age, the AFB1 group average weight (AW), average daily gain (ADG) and average daily feed intake (ADFI) was significantly lower than the control groups, the L.Plantarum group (P<0.05), feed conversion ratio (F/G) is significantly higher than control group, the L.Plantarum group (P<0.05), L.Plantarum group average weight (AW), average daily feed intake (ADFI) and control group had no significant difference (P>0.05). Experiment period (1-28 days of age), AW, ADFI, ADG in AFB1 groups compared with the control group decreased significantly (P<0.05), F/G is significantly higher than control group (P<0.05). AW, ADFI L.Plantarum groups compared with the AFB1 group increased significantly (P<0.05), back to the control level.3. Histopathological:the control group showed normal liver tissue structure, aflatoxin group of liver steatosis and mild liver congestion, and to join in the group of L.plantarum hepatic lesions situation has been restored to control levels.4. organ index:heart, liver index in AFB1 group was significantly higher than control group and in L.plantarum group, significant difference (P<0.05). in L.plantarum group, heart, liver index was decreased significantly (P<0.05).6. AFB1 residues:AFB1 residues in liver, muscle and faeces in AFB1 group was also significantly higher than control group and Lactobacillus plantarum group (P<0.05). Lactobacillus plantarum and the control group had no significant difference (P> 0.05). The residues of AFB1 in the liver, muscle decreased significantly (P<0.05). Faeces residues and aflatoxin B1 group significantly increased compared with control group (P<0.05).In conclusion, Lactobacillus plantarum on aflatoxin have certain adsorption effect, through the adsorption of aflatoxin, complex form AF-bacteria out of the body, increase the AFB1 content in feces, thus reducing the AFB1 in the absorption of animals, Reduce the influence of aflatoxin on broiler growth performance,reduce the AFB1 of animal liver damage, reduce the AFB1 influence on animal organ index, reduce the AFB1 residues in animal liver and muscles. To reduce the harm to animals. |
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