Analysis Of Intestinal Microbial Diversity And Enzyme Activities From Wild And Farmed Chinese Giant Salamander

Giant salamander(Andrias davidianus), amphibia caudata implicit gill salamanders, as Second Class National-Level Protected Animal, is the biggest species of amphibia animals, is also a "living fossil" of animal evolution. Belongs to the carnivorous animals giant salamander, feeding mainly for protein kind of food. Growth and feeding of giant salamander species are greatly influenced by seasonal, giant salamander is the transition of amphibia and crawling class groups, the complex structure of the digestive tract, intestinal diversity of microbial components. The appearance of the intestinal microbial diversity and enzyme production activities can not only provide a basis for wild giant salamander protection, can also provide the basis for artificial breeding giant salamander. To wild and giant salamander breeding are studied in this paper two kinds of samples as experiment material, giant salamander through microbial pure culture technology and free training comprehensive analysis under two kinds of habitat strain analysis of the composition of gut microbes and community diversity. Microorganisms extracellular enzyme methods of two kinds of samples for intestinal bacteria produce extracellular enzyme screening.Wild salamander gut microbial diversity and enzyme activity analysis.Pure culture technology has been used to separate the intestinal bacteria, chooses27F/1492 R amplification, and molecular biology identification to belong to, the experimental results show that gut bacteria can be divided into 2 phyla 6 genera.Application of extracellular enzyme screening medium screening of gut microbes extracellular enzyme activity, the experimental results show that produce protease, amylase,cellulase and lipase strain accounted for 96.51%, 73.26%, 94.19%, 5.82%.Free culture techniques to extract the intestinal contents of total DNA, choose primer799F/1492 R after amplification carrier to connect to the properties and transformation,selection of plasmid primers M13+/M13-screening and sequencing after(PCR-RFLP)analysis, appraisal to genus. The experimental results show that gut bacteria can be divided into 3 phyla 16 genera.Gut archaea from cultivating total DNA, selects the archaea specificity primer amplification after connecting to the carrier and transformation, selection of plasmid primers screening positive clones from sequencing, and identification. The experimental results show that gut bacteria can be divided into 1 phylum 2 class.Farming salamander gut microbial diversity and enzyme activity analysis.Unculture technology has been used to separate the intestinal bacteria, choose799F/1492 R amplification, and molecular biology identification to belong to, the experimental results show that gut bacteria can be divided into 3 phyla 16 genera.Application of extracellular enzyme screening medium screening of gut microbes extracellular enzyme activity, the experimental results showed that the protease, amylase,cellulose enzyme production strain of 89.69%, 73.20%, 86.60%.Unculture techniques to extract the intestinal contents of total DNA, choose primer799F/1492 R after amplification carrier to connect to the properties and transformation,selection of plasmid primers M13+/M13- screening and sequencing after(PCR-RFLP)analysis, appraisal to genus. The experimental results show that gut bacteria can be divided into 4 phyla 15 genera.