Molecular Identification And Cold Tolerance Analysis Of OsC2H2-12 Transcription Factor Transgenic Rice

Rice is one of the most important food crops in the world; it is very sensitive to temperature, and easily to be reduced yield by low temperature. In the northeast of China,chilling injury causes the quite serious loss in rice production. C2H2 zinc finger protein family plays an important role in improving the resistance of plants under various stresses.In this study, the functions of OsC2H2-12 gene in rice seedling stage under low temperature stress were studied by phenotypic analysis, physiological and biochemical indexes detection, gene expression analysis and subcellular localization.The main results are as follows:1. Two over expression transgenic lines were detected by the molecular assay. The result of western blot showed that the OsC2H2-12 gene was expressed in rice, which indicated that the OsC2H2-12 gene was successfully integrated into the rice genome.2. The low temperature germination and chilling tolerance at the seedling stage of OsC2H2-12 transgenic rice were higher than that of control(Kitaake), which indicated that the OsC2H2-12 gene might be involved in the regulation of rice chilling stress.3. In low temperature condition, the expression of OsC2H2-12 gene in transgenic rice was 6 times as high as that of the control, this result indicating the OsC2H2-12 gene played an important role in the regulation of low temperature stress in rice seedling stage.4. Subcellular localization analysis showed that C2H2-12 gene was expressed in the nucleus.5. In low temperature condition, OsC2H2-12 transgenic rice showed higher chlorophyll content, soluble sugar content and soluble protein content, and increased POD activity, indicating OsC2H2-12 transgenic material has certain resistance to cold.