Functional Analysis Of Transcriptional Factor, OsbZIP32for Cold Tolerance In Rice

Chilling damages were distributed widely and affect each growing period of rice.Rice was sensitive to low temperature at germination stage, seedling stage, bootingstage, and heading stage, and its growth and yield are severely inhibited at lowtemperature.The bZIP transcription factor is one of the most widely distributed andconservative protein in the eukaryotic transcription factors. It plays an important rolein the process of abiotic stress response, associated with tolerance to drought, salt,high temperature and low temperature in plant. Using genetic engineering technology,such as transcription factor gene over-expressing or knockdown method, we canmodify the plant to improve their tolerance to biotic and abiotic stress. At present,many bZIP transcription factors have been identified in rice, however, minority genesof bZIP family were participated on low temperature regulation.The molecular biology identification of OsbZIP32transgenic rice was performedin this study. PCR and sequencing results revealed that OsbZIP32has integrated intothe rice genome. Cold stress treatments showed that seed setting rates of transgeniclines have improved significantly comparing with non-transgenic control rice.qRT-PCR analysis demonstrate that OsbZIP32may participate in the regulation ofcold stress tolerance. In addition, cold resistance identification were carried out usingtransgenic lines of27bZIP transcription factors in rice reproductive stage, it willprovide gene resources for understanding cold tolerance mechanism.