The Technology Of Boar Semen Preservation

Sequel to extensive application of artificial insemination techniques, semen preservation technology has also been given more attention. The most commonly used methods of preserving boar semen have been the liquid storage at room temperature and cryopreservation. The production practice of liquid storage at room temperature mainly stored semen in liquid at room temperature however; the application of frozen semen is rare. In order to further improve the sperm utilization efficiency and promotion of boar frozen semen for artificial insemination, the effects of different dilution density on sperm quality and different freezing me thods on sperm quality was investigated in this study. The results show that boar semen diluted with BF5, BTS and Androhep when stored at 17℃ did not have significant difference in terms of sperm quality from first to second day of the experiment. However, from the3 th day to 7th day, dilution in Androhep produced a better effect and result than that of BF5 and BTS.Boar semen preserved in Androhep liquid at17℃ did not show significant difference at different storage density of 2 billion /40 ml, 2 billion /60 ml, and 2 billion /80 ml.Boar semen was frozen when exposed to liquid nitrogen vapor. The freezing effect of Androstar and BF5 as the basis of the freezing liquid had no significant effect. The freezing effect of addition of glycerol at the second dilution was better than the addition of glycerol at the first time.When the boar semen was cryopreserved in liquid nitrogen using Androstar and BF5 as basic freezing fluid, the result also shows no significant difference.Boar semen cryopreserved in particles of dry ice freezing effect was better than liquid nitrogen fumigation, while effects of fumigation with liquid nitrogen cryopreservation was better than- 80 degrees refrigerator freezer.The use of frozen semen in insemination of sows resulted in high pregnancy rate than that of artificial insemination with fresh semen.