| During 2013 to 2015, the bulbs of domestic variety and different imported varieties of Hippeastrum vittatum Herb were used as explants, and the tissue culture propagation experiment was conducted under conventional conditions to study the bacteriostatic agents, plant hormones and other factors on the control of pollution and induction, which determined the best medium for primary culture, subculture, weight gaining and rooting culture. On this basis, starting from the perspective of ecological interspecific competition theory, explored the influence of different antibacterial agents and different varieties of H.vittatum on the bulbs tissue culture propagation of H.vittatum under the environment of open-tissue culture and hydroponic culture, which established a new complete plant regeneration system for H.vittatum. The research showed the following conclusions:(1) Under conventional tissue culture conditions, the explants contamination rate of the nine varieties of H.vittatum, which were inoculated on a medium without antibacterial agent,were more than 90%, and contamination sources were from the endophytic fungi.(2)Under conventional tissue culture conditions, the best medium for primary culture and subculture were all 1/2MS+4.0 mg·L-1 6-BA+2.0 mg·L-1 NAA+50 mg·L-1 AAS+200 mg·L-1 MZ, the medium of 1/2MS+4.0 mg·L-1 6-BA+1.0 mg·L-1 NAA+100 mg·L-1KH2PO4+50 mg·L-1 AAS+200 mg·L-1 MZ was the most suitable for bulb weight increasing and strong seedling, and the best rooting medium was1/2MS+1.0 mg·L-1 NAA+50 mg·L-1 AAS+200 mg·L-1 MZ.(3)In the above preferred medium, for domestic variety H.vittatum, the induction rate of adventitious shoot rose by 99.07%, and the contamination rate was reduced to 31.07%; the contamination rate of subculture was 3.73%, and propagation coefficient was 2.81; weight gain multiplier was 2.90, and the average diameter of little bulbs was 14.67mm; the rooting rate was 100%, rooting number and root length were up 6.25 and 4.13 cm; the survival rate of plantlets was over 95%after transplanted.In the above preferred medium, for H.vittatum of imported varieties, the contamination rates of the 4 varieties (flower peacock, AN, MI, CH) were 89.24%, 14.29%,62.50%,87.50% respectively, which were significantly higher than domestic varieties; in subculture, the propagation coefficient of the three varieties AN, MI, CH were 2.81,4.01,3.17 and the contamination rates were 2.73%,8.54%, 10.06% respectively; weight gain multiples of the three varieties were respectively 2.97,3.01,2.35, and the average diameter of little bulbs were 15.37 mm,16.58 mm, 13.98 mm; the three varieties rooting rate were all 100% in the best rooting medium, which average number of roots were 6.78,7.35,6.01 respectively, and average roots length were 5.12 cm,5.26 cm,4.74 cm; the survival rate of plantlets was over 95% after transplanted.(4)Under room temperature+airy+humidity open environment, combined with mancozeb spray dilution explants treatment, the average contamination rate of primary culture was 69.54%, and the highest induction rate of adventitious shoot was 80.26%, which was significantly higher than the conventional tissue culture result; in subculture, the propagation coefficient of the three varieties AN, MI, CH were 3.81,3.92,3.79 and the contamination rates were 2.93%,3.90%,4.17% respectively; Weight gain and rooting culture was not necessary, whlie the survival rate of plantlets was over 95%after transplanted directly.(5) Under open-hydroponic culture condition, in the best primary culture solution (soil leachate+50 mg·L-1 AAS+200 mg·L-1 MZ), the induction rate of adventitious shoot of explants was 85.32%, and the contamination rate was 24.24%. |
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