Molecular Mechanism Of ABA Pathwayresponse To Zoysia Japonica Being Infected By Rhizoctonia Solani

Japan Zoysia (Zoysia japonica Steud.) is a kind of grass with wide application and strong hardiness. Brown spot caused by Rhizoctonia solani can cause serious damage to lawn grass. In order to seek a more effective way to reduce the impact of brown spot to the lawn industry, through the establishment of Japan zoysia-Rhizoctonia solani vaccination infection system, combining the transcriptome sequencing analysis, this article studies the ABA biosynthesis pathway genes in response, and comes to the following conclusions:1. The transcriptome sequencing showes that ABA biosynthetic pathway in zoysia exists. It originates from glycolysis circulation and the Krebs cycle, ends in terpene compound synthesis and the synthesis of ABA carotenoids, altogether four stages. When the Zoysia plant is infected by Rhizoctonia solani, ABA biosynthesis pathway gene expression showes a total of 37 changes.2. With the response of Zoysia to Rhizoctonia solani in the process of infection, ABA response genes detect that:1) In zoysia glycolytic pathway, a total of four kinds of expressed genes, respectively encoding Phosphate glucose mutase, glucose phosphate glucose-6-phosphate isomerase, fructose phosphate kinase, and fructose diphosphate aldolase, with 8,9,17,12 times expression monomer respectively.2) In zoysia citric acid cycle pathway, a total of 11 kinds of expressed genes, respectively encoding glyceraldehyde 3 phosphate dehydrogenase, glycerate phosphate kinase, phosphate glycerate mutase, enolase, pyruvate kinase, pyruvate carboxylase, pyruvate dehydrogenase, two hydrogen sulfide symplectic acyl acetyl jump enzyme, hydrogen sulfide symplectic two amide dehydrogenase, acetyl coa synthetase, and aldehyde dehydrogenase, with 8,5,18,21,7,8,20,8,10,6,20 times expression monomer respectively.3) In zoysia terpene compound synthesis pathway, a total of 16 kinds of expressed genes, respectively encoding acetyl coenzyme acyltransferase, hydroxymethyl glutaric acid coa synthetase, hydroxymethyl glutaric acid acyl coenzyme A reductase, armour hydroxyl pentanoic acid kinase, armour hydroxyl pentanoic acid phosphate kinase, diphosphate armour hydroxyl pentanoic acid decarboxylase, deoxidization xylulose phosphate synthase, phosphoric acid deoxidizing xylulose reduction isomerase, hydroxy acid methyl phosphate erythritol, sugar alcohol cytidine acyl transferase, methyl red sugar alcohol kinase, erythritol, sugar alcohol methyl diphosphate synthase. hydroxy methyl butene base diphosphate synthase 1, hydroxy methy1 butene base diphosphate synthase2,hydroxyl methyl butene base two phosphoric acid reductase, pentenyl two phosphate isomerase, and method base pyrophosphate synthase, with 8、12、13、8、3、7、1、2、2、3、 3、2、2、6、7 times expression monomer respectively.4) In ABA zoysia carotenoids synthetic pathway, a total of 6 kinds of expressed genes, respectively encoding lycopene beta cyclase, beta carotene hydroxylase, zeaxanthin epoxidase, violet xanthine to epoxy mould, epoxy carotenoids dioxygenase oxygen element dehydrogenase, and Yellow oxygen dehydrogenase, with 1,4,3,1,4,2 times expression monomer respectively.3. In the glycolysis and overall metabolic pathways of ABA synthesis, the metabolic pathways strengthen overallly with disease aggravating. In the Terpene compounds and carotenoid metabolism of ABA synthesis, related gene expression showes a trend of rising in the prophase and metaphase stages of Rhizoctonia solani infecting plants and a trend of decreasing during the latephase. From the comparison of the whole synthetic pathway and damage situation, conclusion is made that the demand to the ABA synthesis is great during the process of Japan zoysia responding to Rhizoctonia Solani infection. In the prevention and treatment of brown spot, glucose phosphate mutase, fructose diphosphate aldolase 19 kinds of key function genes of the four pathways of ABA synthesis can be adjusted to coordinate the synthesis of ABA, and to improve the disease-resistant ability of Zoysia.