The Establishment Of In Vitro Rapid Propagation System From Stem Segment And Dormant Bud In Acer×freemanii ’Autumn Blaze’

In this study, Acer×freemanii ’Autumn Blaze’stem segments and dormant buds of one-year-old seedlings were used as the explants. The effects of different disinfection methods and ways of browning inhibition were studied. The optimum differentiation, multiplication and rooting media were also proposed. In vitro rapid propagation system of Acer×freemanii ’Autumn Blaze’was established, which laid a foundation for the multiplication of Acer× freemanii’Autumn Blaze’and may be helpful to the in vitro propagation of other plants in the genus of Acer. The main conclusions of this study were as follows.1. The most suitable disinfection method for Acer×freemanii’Autumn Blaze’stem segment was 1% NaClO for 4 minutes. The induction rate was higher with the bud scales stripped and leaf primordia exposed in the sterilization of dormant buds. The optimum disinfection method of the dormant bud was when 0.1% HgCl2 was used for 80s. Then the second-best way was 0.1% HgCl2 for 120s. The survival rate of the two methods were both more than 80%2. The optimum differentiation medium for the stem segment was modified MS+0.02 mg/L IBA+0.02 mg/L CPPU; the best proliferation medium was modified MS+0.05 mg/L IBA+0.1 mg/L CPPU; the most suitable rooting medium was 1/2MS+0.05 mg/L IBA+0.1 mg/L NAA.3. The optimum differentiation medium for the dormant bud was modified MS+0.5 mg/L 6-BA+0.02 mg/L IBA+0.05 mg/L ZT; the most proliferation medium was modified MS+ 0.02 mg/L IBA+0.01 mg/L ZT; the most suitable rooting medium was the same to the stem segment.4. PVP has a certain effect on inhibiting browning. With the increase of the PVP concentration, the browning rate of the Acer×freemanii’Autumn Blaze’was significantly decreased. However, transferring to fresh culture medium was more effective, which made the browning rate reduce to 8.9%.5. The differentiation ability of the stem segment was higher than that of the dormant bud. In initiation culture, the induction rate of the stem segment was 95.6% while the induction rate of the dormant bud was just 64.4%. During the process of proliferation, the multiplication factors of dormant bud were higher than that of the stem segments.