| Soil acidification refers soil absorbent composite body to accept a certain amount of exchangeable H+ and Al3+, so that ion leaching alkaline soils, soil pH resulting in reduced process. Biochar loose because the surface long and short features, which can effectively absorb heavy metals in soils. This study aimed to explore the biological carbon Soil acidification refers soil absorbent composite body to accept a certain amount of exchangeable H+and Al3+, so that ion leaching alkaline soils, soil pH resulting in reduced process. Biochar loose because the surface long and short features, which can effectively absorb heavy metals in soils. This study aimed to explore the biological carbon mitigation aluminum ions in the acid soil stress on baccata seedlings rhizosphere physicochemical properties of soil and plant physiological indicators of clear biochar in soil acidification orchard production value and potential is significant.In this study,1-year-old potted Malus baccata Borkh seedlings as test materials, by adding to the potting soil abandoned apple branch biochar made from the outer aluminum ion source, an analog orchard soil acidification alleviates Malus baccata Borkh victory mechanisms of injury and biochar. The results are as follows:Aluminum stress led to Malus baccata seedling rhizosphere soil pH and soil available nutrients decreased, but increased heavy metals in soil, seriously affected plant growth environment. By adding biochar to potting soil can alleviate aluminum stress cause harm to the soil. After first day treatment, the pH of T treatment (With 4 g·kg-1 soil proportion adding AlCl3-6H2O2) was reduced to 5.8. However, the addition of biochar soil pH was 6.9, and the content of available N, P and K were significantly higher than those of Al stress treatment; After fifth day aluminum stress, T and B (With 4 g·kg-1 soil proportion adding AlCl3·6H2O2+5% biochar) rhizosphere soil nitrogen content were decreased by 35.7% and 19.2%, effective K content were 0.90 times and 2.40 times of control CK(Neither adding exogenous aluminum, or add biochar). The content of exchangeable cations such as K+, Ca2+, Na+, Mg2+ and so on in soil is decreased under the aluminum stress, and significant difference with control; Add biochar effectively alleviated aluminum stress on rhizosphere soil damage. The content of heavy metal ions such as Fe3+, Mn2+ Cu2+ and Zn2+ in soil was significantly increased after the addition of aluminum ions. And T the content of exchangeable Mn2+ and Fe3+ was 368.56 times and 1.64 times as many as control. Add biochar treament the content of Mn2+ and Fe3+ was only 153.33 times and 1.02 times as many as control.Aluminum stress caused the destruction of the mitochondrial function of root, embalance of root active oxygen defense system and decline of root vigor. By adding biochar to soil can effectively alleviate the aluminum stress on the plant damage. Aluminum stress increased Malus baccata Borkh root cell membrane lipid peroxidation and the membrane permeability, treatment T compared to control root mitochondrial membrane permeability increased 156.6%, membrane potential 58.1%,MDA content liters high 70.0% after treatment nith day.Add biochar to potted compared with control, mitochondrial membrane permeability increased by 60.9%, membrane potential decreased by 46.8%, MDA content increased by28.7%. What caused of membrane lipid peroxidation was the accumulation of reactive oxygen species such as O2- and H2O2, T and B with the addition of biochar O2- production rate was 2.15 and 2.05 times as much as that of the control CK, H2O2 was control of 5.8 times and 5.0 times, the accumulative of ROS such as O2- and H2O2 was mainly due to the aluminum stress damaged Malus baccata Borkh cell protective enzymes and antioxidant properties of coordination. SOD activity increased rapidly in early and then decreased, CAT was a band of increased and then decreased under aluminum stress, the activity of POD showed an continuously increasing trend in the whole process, and the activity of antioxidant enzyme system was significantly higher than the normal aluminum stress treatments after adding biochar.The root activity of T and B was decreased continuously during the whole process of aluminum stress treatment, and there were CK>B>T in the same processing period.The chlorophyll fluorescence induction kinetics curves of T and B leaves showed obvious changes and appeared more obvious J point after Oms and 2000 ms standardization, which indicated that electronic was blocked from QA to QB and aluminum stress damage the blade side of PS Ⅱ receptors.JIP-test curve fluorescence data analysis showed that were reduced the maximal photochemical efficiency (Fv/FM), photosynthetic characteristics index (Plabs), electron transfer amount of quantum yield(ψEo)and specific activity parameters (RC/CSo), the J point relative variable fluorescence rate(Vj)and K(300 mu s)variable fluorescence Fv accounted for for FJ-Fo amplitude ratio were increased in the whole process. Aluminum stress damaged leaves of Malus baccata photosynthetic mechanism. However, leaf photosynthetic system damage has eased after the addition of biochar to potting soilPot experiment, aluminum stress on Malus baccata Borkh seedlings rhizosphere soil physical and chemical properties of significant influence, the physiological indicators of plant inhibition produced by adding biochar to potting soil, you can ease the stress on aluminum Malus baccata Borkh seedlings rhizosphere soil and environment effect of plant physiological indicators... |
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