Molecular Cloning And Expression Analysis Of Tropomyosin Gene From Grass Carp

This study selected female Ctenopharyngodon idellus and male Squaliobarbus curriculus in distant hybridization between subfamilies, and obtained Ctenopharyngodon idellus (♀) X Squaliobarbus curriculus (♂) F1 fish populations. In the study, we using histology section, digestive enzyme activity and molecular marker detection technology to detect the hybrid fish intestinal tissue morphology and digestive enzyme activity and expression of Peptl, Pept2 in the gut, and comparative analysis of the intestinal structure differences between F1 and its parent, the research results are as follows:1. Comparison of intestinal structureComparative study of three kinds of fish intestinal sections found that the foregut villus height of F1 was between Ctenopharyngodon idellus and red eye trout, higher than Squaliobarbus curriculus villus average height, whereas lower than that of Ctenopharyngodon idellus, and showed significant difference (P< 0.05). F1 goblet cell size and quantity showed no significant difference with Ctenopharyngodon idellus and Squaliobarbus curriculus (P> 0.05). F1 crypt depth mean in Ctenopharyngodon idellus and Squaliobarbus curriculus between, grass carp crypt depth mean was significantly higher than that of F1 and barbel chub (P< 0.05), midgut and hindgut no significant difference (P> 0.05). Three fish contain a certain number of lymphocytes, the foregut lymphocyte mean size followed by F1> Squaliobarbus curriculus> Ctenopharyngodon idellus, and showed significant difference (P< 0.05) and F1 intestinal lymphocyte count was significantly higher than that of Squaliobarbus curriculus and Ctenopharyngodon idellus (P< 0.05), and Ctenopharyngodon idellus and Squaliobarbus curriculus no significant difference (P> 0.05). There was no significant difference between the three kinds of intestinal mucosal lymphocytes (P>0.05).2. The results of intestinal digestive enzyme activityStudy of three kinds of intestinal protease activity found that the protease activity of the intestine in F1 was between Squaliobarbus curriculus and Ctenopharyngodon idellus, in addition to the proteinase activity of the foregut Squaliobarbus curriculus was significantly higher than that of Ctenopharyngodon idellus, Squaliobarbus curriculus foregut and F1 foregut and residual intestinal protease activity had no significant difference (P> 0.05). Amylase activity in the intestine of Ctenopharyngodon idellus was significantly larger than that of Squaliobarbus curriculus and F1 (P< 0.05), F1 intestinal amylase activity slightly larger than Squaliobarbus curriculus, but no significant difference (P> 0.05).3. The expression of Peptl and Pept2 in the gutExpression of Peptl and Pept2 in the intestine was detected by using RT-qPCR. The results showed that Peptl F1 in the gut expression amount in parent between. Squaliobarbus curriculus foregut Peptl expression was significantly higher than that of Ctenopharyngodon idellus and F1 (P< 0.05), F1 in between Squaliobarbus curriculus and Ctenopharyngodon idellus, the Peptl in F1 foregut expression was slightly higher than that of Squaliobarbus curriculus, but the difference was not significant (P>0.05); Peptl in three species of intestinal expression amount from high to low in turn is Ctenopharyngodon idellus> F1> Squaliobarbus curriculus.The expression of Pept2 in Ctenopharyngodon idellus foregut was significant higher than that of F1 and Squaliobarbus curriculus (P<0.05), but there was no difference in the expression between F1 and Squaliobarbus curriculus. The expression of Pept2 in the midgut of Squaliobarbus curriculus is slightly larger than the expression of F1 and Ctenopharyngodon idellus, slightly larger than the Ctenopharyngodon idellus, but there was no significant difference (P>0.05). Squaliobarbus curriculus intestinal expression is slightly larger than the Ctenopharyngodon idellus and F1,but but there was no significant difference (P>0.05).