Preliminary Study Of Coniothyrium Minitans Mycoparasited On Phytopathogen Sclerotinia Sclerotiorum In Metabolomics Level

Coniothyrium minitans is a sclerotial parasite of the plant-pathogenic fungus Sclerotinia sclerotiorum, and have important biological control ability of Sclerotinia diseases. Previous studies mainly focused on molecular biology-related genes described in C.minitans parasitic ability, explore the regulation of parasitic, sporulation capacity mechanism of biological functions. However, few studies have been conducted to further C.minitans as a biological control agent against S.sclerotiorum in metabolites level. In this study, we have used MS based metabolomics tool to explore changes in C.minitans metabolites expression in response to S. sclerotiorum co-culture. The elucidation of the undergoing changes in C.minitans’ metabolism during contacted with S. sclerotiorum is essential for understanding it mycoparasitism mechanism. Our results suggest that C.minitans metabolomics profiles are highly specific with the host fungi and the relative eliciting metabolites may represent a novel approach to develop biopesticides and will pave the way to an avenue of pesticide discovery through targeted activation of silent secondary metabolites gene cluster through co-cultivations of microorganisms.The results are as follows:1. LC-MS based metabolomic analysis technology used to monitor the specific interaction between the filamentous fungi C.minitans with it host S. sclerotiorum in a time series condition. Metabolomics statistic result showed that there is a completely different metabolites change pattern between C.minitans co-culture with its host S.sclerotiorum and S.sclerotiorum alone or C.minitans alone. We find three secondary metabolites Macrosphelide A, Benzenediol, 5-Aminopentanoate were detected in C.minitans contacted with host pathogenic fungi, and co-cultured with 2 days is the hightest accumulation point. Result showed that fungi communication can significantly regulate the biosynthesis of secondary metabolites ability and mycoparatsitism involved the interaction of secondary metabolites.2. C.minitans is an obligate sclerotial mycoparastie of the plant-pathogenic fungus S.sclerotiorum, while it is unable to parasitize B.cinerea, a closely related fungus of S.sclerotiorum. Result showed that C.minitans produced a variety of metabolites when it contacted with phytopathogen fungi, but there is a completely different metabolites change pattern between C.minitans with it host S.sclerotiorum and not it host B.cinerea. Data suggested that lipid signaling metabolitesmay play an important role in C.minitans unique metabolic cross talk for it obligate mycoparasitism ability.3. A computational algorithms called mummichog, combined the metabolic model Saccharomyces cerevisiae was used to explore changes in C.minitans metabolites expression during growth on mycelia of S. sclerotiorum and B.cinerea.Differentially expressed metabolites modules were found in C.minitans under the two different conditions. And various metabolites of biotechnological value in pathways level such as zymosterol biosynthesis, sphingolipid recycling and degradation(yeast) were involved in fungi communication. This unique metabolites change may involved in C.minitans obligated mycoparasitism ability.