Acylation Of Blueberry Anthocyanins And Its Proliferation Inhibitory Activity Of Hep G2 And Caco-2 Cells

Anthocyanin as a natural active substance has a variety of physiological health functions, but it has some problems that restrict its application range, such as poor stability and low solubility in oil, which due to its large molecular polarity. In this paper, the anthocynin was modified by acylation, the antioxidant capacity of acylated anthocyanin was explored by extracorporeal antioxidant model, and then cell experiment was carried out to identify whether the acylated anthocyanin could inhibit the proliferation of cancer cells. The results were as follows:(1) Confirming that Cyanidin-3-O-galactoside was existed in bluberry anthocyanin by LC-MS. The preparation method of Laurie acid acylated anthocyanin was determined:a. synthesis reaction of lauric acid acylated group; b. acylation of lauric acid acylated group and bluberry anthocyanin; c. the progress of the reaction was monitored by LC-MS; d. the acylated product was separated and purified by Prep-LC. The result of LC-MS showed that a substance m/z=632.2653 existed in the acylated product, and the acylation rate was 28.94%. The total phenol contents of acylated anthocyanin and bluberry anthocyanin were 117.3±7.4 mg/g and 10.6±4.3 mg/g, there was a significant increase after acylation.(2) For inhibition of lipid peroxidation and scavenging assay of ABTS+ and DPPH, the antioxidant capacity of acylated anthocyanin was better than bluberry anthocyanin; but for the scavenging assay of O2-. and OH-, the antioxidant capacity of acylated anthocyanin was lower than ascorbic acid. For the inhibition of lipid peroxidation experiment, the IC50 values of acylated anthocyanin and bluberry anthocyanin were 121.7 and 201.3 μg/mL; for the scavenging assay of ABTS+. experiment, the values were 20.1 and 42.3 μg/mL; for the scavenging assay of DPPH.experiment, the values were 28.9 and 40.6 μg/mL; for scavenging assay of O2-. experiment, the values were 19.1 and 33.4 μg/mL; for scavenging assay of OH.experiment, the values were 125.6 and 141.2μg/mL.(3) The MTT results showed that both acylated anthocyanin and bluberry anthocyanin could inhibit the proliferation of Hep G2 and Caco-2 cells, and the inhibitory effect of acylated bluberry anthocyanin was better than bluberry anthocyanin, and the inhibitory effect of Hep G2 cells was better than Caco-2 cells. The IC50 vahues of acylated anthocyanin for Hep G2 cells and Caco-2 cells were 0.415 and 0.645 mg/mL, the value of bluberry anthocyanin were 0.685 and 2.634 mg/mL.