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Under Water Stress Carbohydrate Soaking On Germination Of Maize And Rhizosphere Microorganisms

Posted on:2017-02-25Degree:MasterType:Thesis
Country:ChinaCandidate:S S SunFull Text:PDF
GTID:2283330485972422Subject:Agricultural resource utilization
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Drought conditions have severely affected the growth of maize, thus affecting the growth of maize, quality. In this experiment, three kinds of exogenous sugars:pullulan, starch, glucose corn seed soaking seeds Shen Wo 201, in four kinds of aridity at (0,5%,10%,15% PEG-6000 concentration) stress study maize seed germination and seedling growth. By studying the germination potential, seedling height, root length, seedling dry weight and root dry weight was measured and statistical analysis, as well as pot experiment to study different water conditions, on maize rhizosphere environment, by measuring the rhizosphere changes in microbial activity in the soil and rhizosphere, the results showed that:Under water stress, with pullulan, starch, glucose solution after soaking, to varying degrees of ease drought on the inhibition of seed germination. In 15% PEG stress, the starch and glucose promoted the seed germination rate. In four moisture conditions, soaking significantly promote seed germination potential, the role of a concentration of 30mg/L of the best, the best of glucose.With pullulan, starch and dextrose solution soaking, can improve corn germ length, root length, and dry weight of radicle and germ. Under drought stress, glucose is more conducive to the growth of corn germ; in normal water when compared to the other two exogenous glucose sugar, in favor of embryo dry weight accumulation, but in drought stress, starch and pullulan on a greater role in the accumulation of the dry weight of the germ. In normal water, three kinds of substances 10mg/L could promote the growth and dry matter accumulation of root length, under drought stress conditions and accumulate starch and pullulan more conducive to root growth and root dry matter.The number of soil bacteria, fungi, actinomycetes with increasing water stress gradually decreases. By soaking treatment can be varying degrees of ease water stress on soil microbial inhibition. When normal water, glucose and starch can significantly increase the number of bacteria and actinomycetes, the optimum concentration is 20mg/L, the role of fungi is not significant; during drought stress, starch and glucose could significantly promote the growth of actinomycetes starch growth of bacteria in the best glucose growth of fungi on the best.Water stress inhibited soil urease and invertase activity by seed soaking treatment, to alleviate the drought inhibition of enzyme activity in the soil. In normal water, glucose can significantly promote urease and invertase activity, the optimum concentration of 10mg/L, starch and pullulan role of urease and invertase is not significant; in mild drought, three kinds of seed soaking substances can significantly promote the urease activity of glucose urease of significantly greater than the starch and pullulan, starch and glucose significantly increased invertase activity, pullulan sucrose enzyme activity is not significant, the best urease concentration of 20 mg/L, the optimal concentration of sucrose enzyme was 10mg/L; when severe drought, three kinds of substances can significantly increase seed soaking urease and invertase activity, and the role of glucose greater than starch and pullulan, the optimum concentration of urease is 10mg/L, the optimal concentration of sucrase was 30mg/L.
Keywords/Search Tags:Water stress, carbohydrate, Maize, Seed soaking
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