The Chromosome Interaction Between Pseudo-Rabies Virus And Host In 3D Level

Chromatin conformation capture technology enable people to find out that the chromosomes are not haphazard arranged in nucleus, but occupy different territories of the nuclei and exercise their different functions. There are a lot of interactions between each chromosome. Many experiments have proved that the 3D genome structure plays an important role in the cell function exercise.Pseudo-rabies virus’ s nucleic acid is a linear double-stranded DNA, which will quickly form a circle after entering the nucleus, and replicates in a rolling circle way. The study on the interaction of PRV with host goes deeper and deeper, but it still lacks precise understanding on the relative position between them in space. We speculate that the PRV will act as a part of the host genome after it get into nuclei to interact with host genes in 3D level to regulate cell functions for its own good.In the present study, we used chromatin conformation capture technology to explore the spatial relationships between the PRV genome and the host genome. The results are as follows:1.Through comprehensive analysis of existing methods, we constructed genome interaction library of PRV infected host cells. By electrophoresis and sequencing analysis, the library was found to be consistent with our expectation and can be used in the downstream analysis.2. After building the library, through the next generation sequencing and bioinformatics analysis, we found a strong interaction between PRV genome and host genome with a strong experimental reproducibility. We illustrate that the results obtained are not due to experiments’ random errors, but the exact situation.3.By real-time PCR, we found that the interaction between the genomes has some biological significance. Host genes interact with the same viral genome point are up-regulated or down-regulated in unison, which is consistent with the current 3D genome research. It is suggested that the genes in a same region are either suppressed or activated simultaneously.4.In addition, we found a spatial structure in the interior of the viral nucleic acid. Fragments of the viral genome interact with other positions with different frequency. Some locations have strong interactions with many locations, indicating that these points are relatively close to other locations and play important roles.The study reveals the interaction between virus and host genomes in 3D level, offering a theoretical basis for people to further explore the interaction between virus and host. It also provides a new perspective for the future prevention of virus infection, through blocking the interaction between the virus and the host genome to hold back the viral genomic integration, and thus to clear those latent virus.