The Effects Of Three Chiness Herbal Medicine On The Tilapia(Oreochromis Niloticus)Under Low Temperature Stress

The purpose of this study was to investigate the effect of Rhodiola Sachaliensis, Astragalus and Zingiber ojjicinale Rosc aqueous extract powder on tilapia anti low-temperature. The survival rate, blood biochemical indexes and antioxidant indexes of the fishes in each group was determined at 30℃、25℃、20℃、15℃ and 10℃.The main results were showed as below. 1. Function analysis of Rhodiola Sachaliensis on improving low-temperature tolerance characteristic of TilapiaAn experiment was conducted to study the effect of Rhodiola Sachaliensis aqueous extract powder on the tilapia under low-temperature. The fishes were divided into four groups: no additives group(control group, NC), R. achlinesi low dose group(0.1%), R. achlinesi mid dose group(0.2%) and R. achlinesi high dose group(0.4%). The fishes were fed at 30℃ for 2 weeks followed by the experimental temperature dropped from 30℃ to 10℃ with the rate of 0.5℃·d-1. The survival rate, blood biochemical indexes and antioxidant indexes of the fishes in four groups were determined at 30℃, 25℃, 20℃, 15℃ and 10℃. The results showed that the survival rates of the all adding R. achlinesi groups were significantly higher than that of the control group. At 10 ℃, compared with the control group, high density lipoprotein-cholesterol, glucose, triglycerides, cholesterol, nitric oxide, malonaldehyde content of fishes in R. achlinesi supplement groups reduced, the differences were statistically significant(P<0.05); meanwhile superoxide dismutase levels and glutathione peroxidase activities in liver of fishes were significant increased with the increasing R. achlinesi level(P<0.05). In conclusion, R. achlinesi can play a positive role in resistant Low temperature of tilapia. 2. Function analysis of Astragalus on improving low-temperature tolerance characteristic of TilapiaIn this study, the effect of Astragalus aqueous extract powder on tilapia anti low-temperature was observed. The tilapia were divide into four groups: no additives group(control group, NC), Astragalus low dose group(0.1%)、Astragalus mid dose group(0.2%), Astragalus high dose group(0.4%). And the fishes were fed at 30℃ for 2 weeks followed by the experimental temperature dropped from 30℃ to 10℃ with the rate of 0.5℃·d-1. The survival rate, blood biochemical indexes and antioxidant indexes of the fishes in four groups were determined at 30℃、25℃、20℃、15℃ and 10℃. The results showed that the survival rate of the all adding Astragalus groups was significantly higher than the control group. At 10℃, compared with the control group, high density lipoprotein-cholesterol, glucose, triglycerides, cholesterol, nitric oxide, malonaldehyde content of fishes in Astragalus supplement groups reduced, the differences were statistically significant(P<0.05); superoxide dismutase, catalase and glutathione peroxidase activities of fishes in Astragalus supplement groups increased, the differences were statistically significant(P<0.05), with greater effect at higher supplemental level. In conclusion, Astragalus can play a positive role in resistant cold stress of tilapia. 3. Function analysis of Zingiber ojjicinale Rosc on improving low-temperature tolerance characteristic of TilapiaIn this study, the effect of Zingiber ojjicinale Rosc aqueous extract powder on tilapia anti low-temperature was observed. The tilapia were divide into four groups: no additives group(control group, NC), Zingiber ojjicinale Rosc low dose group(0.1%)、Zingiber ojjicinale Rosc mid dose group(0.2%), Zingiber ojjicinale Rosc high dose group(0.4%). And the fishes were fed at 30℃ for 2 weeks followed by the experimental temperature dropped from 30 ℃ to 10℃ with the rate of 0.5℃·d-1. The survival rate, blood biochemical indexes and antioxidant indexes of the fishes in four groups were determined at 30℃、25℃、20℃、15℃ and 10℃. The results showed that the survival rate of the all adding Zingiber ojjicinale Rosc groups was significantly higher than the control group. At 10℃, compared with the control group, glucose, triglycerides, cholesterol, nitric oxide, malonaldehyde content of fishes in Zingiber ojjicinale Rosc supplement groups reduced, the differences were statistically significant(P<0.05), and high density lipoprotein-cholesterol showed no difference; superoxide dismutase and glutathione peroxidase activities of fishes in Zingiber ojjicinale Rosc supplement groups increased, the differences were statistically significant(P<0.05), with greater effect at higher supplemental level. In conclusion, Zingiber ojjicinale Rosc can play a positive role in resistant cold stress of tilapia.