Identification Of Major QTL For Adult Plant Resistance To Stripe Rust In Wheat Landrace Caoxuan 5 And Lantian 1

Wheat stripe rust is one of the most destructive diseases on in the world. It caused by Puccinia stiiformis f. sp. tritici. And it is a typical obligate and air-borne fungal disease.China is the biggest independent endemic area of stripe rust. Resistance of main cultivars lost about 7 times in China. The utilization of diverse genetic background of resistance genes and wheat materials could make the rust races slow down, and it also made the time of application longer. Approaches to control stripe rust, the implementation of resistance is the most low-cost, effective, and environmentally-friendly.In this study, 6 results were got:1. Caoxuan 5 was susceptible(IT 7–9) when tested with all 6 races at the seedling stage,but resistant(IT 2–3) at the adult plant stage. M169 was highly susceptible(IT 9) at both the seedling and adult plant stages in all tests. The results confirmed that Caoxuan 5 has APR to stripe rust.2. Construct a mapping population of the F2 family with which derived from the cross of Mingxian 169 and Caoxuan 5. The genetic linkage map was established with RGAP and SSR molecular markers. The F3 lines of Mingxian 169/Caoxuan 5 were evaluated at Tianshui(Gansu province) and Yangling(Shaanxi province) during the 2013-2014 cropping season.The disease severity and infection type were recorded for the QTL analysis.3. Two APR QTL of Caoxuan 5 conferring stable resistance were detected on 3BS and6 AL chromosome by QTL IciMapping 3.3. The QTL on 3BS was designated as QYrcx.nafu-3BS and flanked by molecular marker Xgwm533.2 and Xgwm493 which can explain 17.3%-38.1 % and 18.2%-29.9 % of the phenotypic variance at Yangling and Tianshui. The QTL on 6AL was designated as QYrcx.nafu-6AL and flanked by molecular marker Xwgp5471 and Xwgp5576 which can explain 9.7%-13.0 % and 10.8%-19.8 % of the phenotypic variance at Yangling and Tianshui.4. Lantian 1 was resistant(IT 0; 1; 1+) when tested with all races at the seedling stage and adult plant stage under high temperature, but susceptible(IT 3-4) under normal temperature.M169 was highly susceptible(IT 4) at both the seedling and adult plant stages in all tests. The results confirmed that Lantian 1 has high-temperature resistance to stripe rust.5. Construct a mapping population of the F2 family with which derived from the cross of Mingxian 169 and Lantian 1. The genetic linkage map was established with RGAP and SSR molecular markers. The F3 lines of Mingxian 169/Lantian 1 were evaluated at Tianshui(Gansu province) and Yangling(Shaanxi province) during the 2013-2014 cropping season.The disease severity and infection type were recorded for the QTL analysis.6. One HTAP QTL of Lantian 1 conferring stable resistance was detected on 1BL chromosome by QTL Ici Mapping 3.3. The QTL on 1BL was designated as QYrlt.nafu-1BL and flanked by molecular marker Xbarc61 and Xgwm403.