The Effect Of H.villosa 1VS On Wheat And Agronomic Comparison Of Its Main Characters

Annual H.villosa has a number of good agronomic traits, wheat quality improvement of valuable genetic resources. After years of research scholars, it has a gene encoding H.villosa many excellent traits positioned on the respective 1V-7V chromosomes. H.villosa 1V short arm of chromosome containing the gene coding for gliadin, and can significantly increase the strength of wheat gluten, modified wheat quality. This study was designed according to the NCBI database H.villosa α- gliadin gene and the development of two pairs Haynaldia 1VS molecular markers, the use of newly developed molecular markers Xinong 979, Xinong 538 Xinong 558, West 556 farmers, Zhoumai18 and Xiaoyan 22 varieties BC4 backcross population screening to identify 1VS generations, and for six common wheat and turn fertile plant height, peduncle length, ear length, number of tillers, spikelets, grain weight and grain number seven kinds of agronomic traits were significantly different assessments. The results are as follows:1. According to the NCBI database H.villosa α- gliadin gene specific primers were designed 132 pairs, the successful development of the next two pairs can be detected in wheat background Haynaldia 1VS chromosome marker F2 / R3 and F8 / R3.2. Using two pairs of primers Xinong 979, Xinong 538 Xinong 556 Xinong 558, Zhoumai18 and Xiaoyan 22 backcross breed(T137, T141, T143, T147, T149 and T155) BC4 Progeny(each 20) were identified 1VS markers. Results in the T137 is not detected H.villosa 1VS chromosomes in the T141 is not detected H.villosa 1VS chromosomes in T143 of all detected H.villosa 1VS chromosome detected in T147 contains H.villosa 1VS chromosome 7 detected in T149 contains H.villosa 1VS chromosome 10, containing all detected H.villosa 1VS chromosome in the T155.3. Have been identified Haynaldia 1VS of backcross breed T143, T147, T149 and T155 recurrent parent and its corresponding seven agronomic traits t distribution test and evaluate the difference was significant. The results of its height, peduncle length, tiller number and grain weight comparison significant differences; ear length and grain number comparison the difference was not significant; spikelets Xinong 556(T143) and Zhoumai 18(T149) contrast significantly different West Agriculture 558(T147) and Xiaoyan 22(T155) comparing the difference was not significant). Actual performance concrete: Wheat plant height, peduncle length increased, the number of tillers and increased grain weight increase.4. Four varieties of common wheat and backcross breed t-distribution detecting differences in seven of its significant agronomic traits were evaluated, see H.villosa 1VS chromosomes on common wheat agronomic traits. Results for plant height, peduncle length, tiller number and grain weight comparison significant differences; spike length, number of spikelets per spike and contrast the difference was not significant.