| Syringa pinnatifolia is a specific endangered tree species of national protection categories 3, the only a pinnately compound plant of Syringa. Its flower is beautiful with aromatic and can be used for excellent ornamental flowers shrubs. Its root, stem and branch of S. pinnatifolia has high medicinal value used as medicine. But S. pinnatifolia is still living in the wild, the quantities of its populations discount continuously. Setting rate and germination rate of seed were low in nature condition, therefore establishment of S. pinnatifolia tissue culture breeding system, cultivation a certain number of seedling, researching genetic diversity and application in landscaping, will have an important significance to increase landscape tree species, provide drug raw materials and protect germplasm resources of S. pinnatifolia. The buds and seeds of S. pinnatifolia, were used as the test materials in the research to study the influence of different disinfection methods, concentration combinations of sucrose and hormone on primary culture of seeds, combinations of basal media and hormones on shoots proliferation and rooting by plant tissue culture method. The genetic diversity of 6 populations of S. pinnatifolia was analyzed by using amplified fragment length polymorphism(AFLP) technique, in order to provide theoretical basis for tissue culture propagation, discussion on the causes of the endangered, germplasm resources protection and exploitation of S. pinnatifolia. The results of research were as follows:(1) The shoots were the suitable explants to establish a sterile system. The appropriate disinfection methods were 75% ethanol for 30 s, 0.1% mercuric chloride for 7min, the best proliferation media was MS containing 5.0mg/L 6-BA and 0.01mg/L IBA, the incisions of proliferation produced callus, but that couldn’t be induced to root on many types of culture media.(2) The optimal initial, proliferation and rooting media for seed was MS medium containing 3.0mg/L 6-BA and 0.1mg/L IBA, MS medium containing 7.0mg/L 6-BA and0.05mg/L IBA and WPM medium containing 2.0mg/L IBA respectively. The root rate,number and average lenth was 60%, 4 and 3.17 cm respectively. The seed was suitale to establish a tissue culture system of S. pinnatifolia.(3) 5 pairs were screened from 64 pairs of primer combinations, 171 DNA bands were amplified, 153 of them were polymorphic, the percentage of polymorphism bands(PPB) was89.47%.(4) The result of AFLP analysis showed that S. pinnatifolia had higher genetic diversity at species level. The PPB, Nei’s gene diversity index(h), Shannon information index(I) were63.84%, 0.2095, 0.3153 respectively. S. pinnatifolia had lower genetic diversity at population level. In the total variation, 23% variation comes from among the S. pinnatifolia populations.The Nm value was 2.1916, showed that the populations of S. pinnatifolia had gene exchange at some degree.The unweighted pair group method with arithmeticmean(UPGMA) showed that there was no significant correlation between genetic distance and geographical distance among populations of S. pinnatifolia.(5) The corresponding conservation and utilization strategy were put forword according to the characteristics of S. pinnatifolia. The on-situ conservation strategy was protecting population in Yinchuan area priority. The ex-situ protection measures were choosing adequate moisture and summer temperature suitable area as ex-situ protection zone, collecting seeds widely from the owner of the genetic diversity of maximum population. Carry out the breeding technology, seed germination, extract and its medicinal value research. Carry out the research on propagation technique, seed germination characteristics, extracts and their drug value of S. pinnatifolia. |
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