| It was an effective way in production for the variety source ofAconitum carmichaeli Debx. by breeding systematically and hybrid breeding based on collecting universally germplasm; as well as through tissue culture in vitro, constructing system of rapid propagation seedlings, which proved an convenient and profitable assessment to solve difficlty of seedlings.In this research, germplasm resources ofAconitum carmichaeli Debx. were studied by RAPD and AFLP molecular identification techenique respectively, also tissue culture in vitro was performed ,the results as follow:1 With tissue culture method,15,9 and 12 medium of hormones combinations were developed to induce callus of different explant derived from Aconitum carmichaeli Debx., induced the differentiation of cluster buds from callus and induced the rooting of regenerated seedlings,respectively. For Aconitum carmichaeli Debx., the ideal culture media for callus induction of stem-tips and stem segments was identified to be MS＋NAA 0.2mgL-1+6-BA 2.0 mg/L＋PVP (or AC) 3.0 g/L. The ideal culture media for callus induction on leaves was identified to be MS＋NAA 0.2 mg/L＋6-BA 4.0 mg/L＋PVP (or AC) 3.0 g/L. The ideal culture media for callus propagation and cluster buds induction was identified to be MS＋NAA 0.2 mg/L＋6-BA 2.5 mg/L＋LH200 mg/L＋PVP3.0 g/L and MS＋NAA 0.1 mg/L＋6-BA 2.0 mg/L＋LH 200 mg/L＋PVP 3.0 g/L. The ideal culture media for rooting induction was identified to be MS＋IBA1 mg/L＋PVP 3.0 g/L.2 Industrialized rapid propagation of seedlings could be realized by in vitro tissue culture with different explant ofAconitum carmichaeli Debx. such as shoot tips, leaves and stem segments with or without axillary buds.3 Total gemomic DNA were extracted from sampled materials by improved 2%CTAB method, and RAPD markers were amplified to detected the sampled 23 germplasms by 15 random primers. 15 primers produced 242 DNA segments, among which 202(83.5%) bands were polymorphic. On average 13.47 polymorphic segments were amplified by each primer. The results of cluster analysis showed that all of the sampled germplasms could be differentiated by RAPD markers. The similarity coefficients ranged from 0.155～0.940 in RAPD analysis, which revealed that different materials of Aconiturn carmichaeli Debx. had rich genetic diversity. (ii) On the similarity coefficients of 0.582,the sampled 23 germplasms were classified into 9 groups by clustering analysis. (iii) RAPD markers could be one effective method to construct DNA fingerprintings of Aconitum carmichaeli Debx.4 AFLP marker was firstly employed to study germpasm of Aconitum carmichaeli Debx. 12 combinations of AFLP primers was applied to analysed on the polymorphism of genomic DNA extracted from 23 Aconitum carmichaeli Debx. tested ,Using statistical software of NTSYS-PC Version 2.1 to calculate the data from AFLP,it was expected to obtain genetic similarity coefficint and construct dendrogram.the results showed that 1238 ffagements of DNA were amplified by the selected 12 combinations from AFLP primers,among which 1019(82.3%)fragements was polymorphism; The similarity coefficients ranged from 0.336～0.910; On the similarity coefficients of 0.68, 23 sampled materials were classified into 11 groups by clustering analysis. Of which 10 sampled was clustered into one group,6 sampled materials in rest was clustered into another group in light of 2 sampled materials respectively, the other was clustered by itself.The results of AFLP marker suggested much more genetic diversity of Aconitum carmichaeli Debx. from different sources,nevertheless,the message from neighboring- join poorly correlated with the message from differency of secondary metabolites, it was likely that genetype and environment made this differenc through affecting the production of secondary metabolites.5 The results of RAPD and AFLP showed rich genetic diversity among sampled materials in different sources; AFLP profile could be identification marker for each variety or cultivar ofAconitum carmichaeli Debx.
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