Optimization Of Fermentation Conditions And Formulationas Of Bacillus Amyloliquefaciens Y-S-Y12 Strain On Biocontrol Of Cylindrocarpon Destructans

Biocontrol with Bacillus amyloliquefaciens strain Y-S-Y12 ginseng rust rot bacteria have a strong antibacterial effect, through the Y-S-Y12 concentrate on optimizing fermentation experiment with different culture conditions can be determined Y-S-Y12 fermentation concentrate the number of days in culture were 3 d, a temperature in the range of 28℃-33℃, the number of revolutions was 150 r· min-1 liquid volume was 125 mL (250 mL flask), initial pH value of 6 for NA test fermentation medium is the medium, the ginseng rust rot pathogen inhibition best;For Y-S-Y12 fermentation concentrate of different nutritional conditions were tested to determine the Y-S-Y12 fermentation broth the best carbon source is glucose and beef extract nitrogen source, inorganic salt is best potassium phosphate dibasic.Articles on different antagonistic bacteria (YS-Y12, YBN13, YBN18) of the dosage form has been studied using talc, kaolin and activated charcoal made of agents, antagonistic bacteria viable count of rust and rot pathogen control effect of ginseng were indoor testing, results detect different forms of antagonistic bacteria should be used as:YS-Y12 strain formulations using talc and kaolin; YBN13 strain formulations using talc, kaolin and activated carbon; YBN18 strain formulations using talc and kaolin.Formulations of agents results will not be one of antagonistic bacteria and dosage of agents and field agents were mixed between prevention test, the test results: Y-S-Y12 strain and Y-S-Y12 strains YBN 13 strains, YBN18 strains different Mixtures of ginseng rust rot Disease Prevention and ginseng growth promoting effect more obvious control effect reached 72.04%～77.42%.