Biological Activity And Effects Of Sabina Vulgaris Essential Oil On Sublethal And ATPase Activities Of Paratrioza Sinica

As one of’three pests of Lycium barbarum’,Paratrioza sinica lead to the growing areas caused serious economic loss. The insecticidal effects had been reported for many times about Sabina vulgaris essential oil, and it was highly selective, harmless to human and animal, pollution-free to the environment. Therefore, the toxicity activity, sublethal effects and mechanism of action were studied, It whether can replace high poison pesticides to biological control on P. sinica have a great significance. The contact toxicity and fumigation of S. vulgaris essential oil on the adult and nymph of P.sinica were determined by impregnation, pesticide-membrane method, fumigation method. The repellence of S.vulgaris essential oil on the adult of P. sinica was determined by Y-tube olfactometer. Lycium barbarum seedlings was spraied with the 100μl/L concentration solution of S. vulgaris essential oil.5 pares of adults were transferred to the seedlings, determined the sublethal effects. The activities of Na-K-ATPase, Ca-Mg-ATPase were determined on the adult of P. sinica in vitro. The results are as follows:1. Toxicity activities of S. vulgaris essential oil had effect obviously, it was the strongest on the first instar nymph of P. sinica, the LC50 value was 1162.1μl/L. The contact toxicity of S. vulgaris essential oil decreased as the increase of P. sinica nymph instar. The fumigation effects was stronger than that of con tact toxicity, dose effect was direct proportion relationship on adults. The repellence effect of the oil on adults was significant, and the repellent rate was 53.1%.2. P. sinica had a significant influence on the sublethal effects by feeding the spraied seedlings. The fecundity of P. sinica was not effected significantly by S. vulgaris essential oil, but the oil reduced egg hatching rate, longed egg and nymph development durations. S. vulgaris essential oil was toxicity to P. sinica and also had some sublethal effects on P. sinica. Compared with controls, the egg hatching rate was reduced by 23.2%, the highest egg stage extended to 1.3 d, nymphs period extended the highest to 7.0 d.3. S. vulgaris essential oil had a significant influence on Na-K-ATPase and Ca-Mg-ATPase on the adult of P. sinica in vitro. S. vulgaris essential oil had remarkable inhibiting action on Na-K-ATPase on the adult of P. sinica in vitro. With the increase of concentrations of S. vulgaris essential oil, the inhibiting action were increased, the highest concentration of the corresponding enzyirs inhibition rate of 37. activation at low concentration and inhibiting action at high concentration on Ca-Mg-ATPase in vitro. With the increase of concentration of S. vulgaris essential oil, he inhibiting action were receded. When the concentration more than 2000μL/L, the tivity of Ca-Mg-ATPase was significantly inhibited. Inhibition and essential oil concentrations was positively proportional relationship. With the increase of concentrations, the inhibition had been enhanced, the highest concentration of the corresponding enzyme inhibition rate of 31.96%.