| Fibroblast growth factor 10(FGF10) is a member of the FGF superfamily. It exhibits diverse biological functions and some abilities to promote wound healing, the regrowth of hair, the proliferation and differentiation of stem cells and cure the facial telangiectasis. Oil bodies, obtained from oil seeds, could be used to produce heterogonous proteins with brief purification steps and lower production costs. Safflower is self-pollinating with low out-crossing habits, and is well adapted to the semi-arid conditions of the tropics and subtropics, which facilitate to manufacture on a large scale with high yield. Moreover, safflower seeds contain low amounts of water and protein kinase, facilitating maintenance of heterogonous protein stability, and transport in room temperature.Hence, recombinant human FGF10(rh FGF10) was expressed in safflower(Carthamus tinctorius L.) seeds using oilbody-oleosin technology. A plant expression vector, pOTBar-oleosin-rhFGF10, was introduced into safflower using Agrobacterium tumefaciens transformation, and mature safflower plants were obtained by grafting. Through PCR, SDS-PAGE and Western bloting analysis, it was confirmed that oleosin-rhFGF10 was successfully transformed and expressed in safflower seeds and inherited to the T3 generation. And the expression levels of rhFGF10,with 0.14% of the total oilbody proteins, was measured by Elisa. Moreover, MTT assays demonstrated that oil bodies expressed oleosin-FGF10 had a dose-dependent effect on cellular proliferation. When the oil bodies expressed oleosin-rhFGF10 was smeared to C57BL/6 mice in a uniform telogen state, anagen hair growth was conmpared between the oil bodies expressed oleosin-rhFGF10- and PBS-administered mice. The results showed that the oil bodies expressed oleosin-rhFGF10 could promote hair growth. In conclusion, this may provide a method of producing oleosin-rhFGF10, and help us meet the increasing pharmacological demands for the protein. |
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