Cloning And Expression Analysis Of Peanut Genes Responsive To Ralstonia Solanacearum

China is the largest peanut planting, export country in the world, and peanut is an important cash crop in our country. Bacterial wilt(BW) disease caused by Ralstonia solanacearum is the most devastating bacterial disease in peanut, which severely limits the peanut production of our country and many countries in Southeast of Asia. Rotation, chemicals and biological controls can prevent the occurrence of bacterial wilt disease in a certain extent. However, these methods are less feasible because of land resources wasting and the cost increase of planting. So far, many researches have been carried out at home and abroad. It illustrates that breeding and utilization of resistant varieties is the fundamental measures to control BW of peanut. Therefore, the depth research of peanut genes responsive to Ralstonia solanacearum is imperative, as well as improvement and cultivation of high resistance to BW of peanut varieties is equally important.There are two main conventional approaches in peanut breeding for BW resistance: one is screening the BW resistance of the peanut cultivars; the other is crossing BW-resistance genotypes with the cultivated peanuts. But the conventional breeding methods cannot satisfy production development, due to the limitation of genetic base of resistance sources and the selection methods. In recent years, with the development of molecular biology, molecular markers and isolation of genes related to disease resistance are widely use in crop genetic improvement, which may help to accelerate the breeding process of BW-resistant peanut cultivars.This study involves the excavation of peanut genes responsive to Ralstonia solanacearum and cultivation of the BW resistant varieties(lines) in peanut. Main contents and results are as follows:1. Genefishing technology was used to isolate and obtain peanut genes responsive to Ralstonia solanacearum, and the cDNA and DNA sequences of these genes were cloned successfully by RACE from Arachis hypogaea cultivar Ri Hua 1. In this study, a total of 3 related genes cDNA were obtained, and the Blastn results show that these genes may be involved in signal transduction, transcriptional regulation, protein synthesis, related disease, basal metabolism and so on in the plant.2. This study constructed over-expression vector, antisense expression vector, and prokaryotic expression vector for these related genes, of which CXIP4-1 gene was transformed into peanut plants, and T1 transgenic peanut plants will be screened to identify its function.3. The analysis of tissue expression and stress expression of 3 related genes in Arachis hypogaea cultivar Ri Hua 1 were conducted by qRT-PCR, for preliminarily testing the function of these 3 genes in responsive to Ralstonia solanacearum in peanut.4. To identify the F1 true hybrids of peanut cross combinations, SSR and AhMITE molecular markers were employed, which may help to hasten the breeding process of BW-resistant peanut cultivars(lines).