Study On Optimization Of Fermentation And Enzymolysis Conditions And Their Effects On Feedstuff Quality Of Maize Stalks

In this study, the influence of fermentation and enzymolysis of maize stalks were investigated, and the best process were optimized. According to the crude protein content, crude fiber degradation rate, NDF degradation rate, ADF degradation rate,hemicellulose degradation rate, cellulosedegradation rate, lignin degradation rate,total acid content, total sugar and reducing sugar content of maize stalks, the effects of separate fermentation, separate hydrolysis and combination treatment on feedstuff quality of maize stalks were investigated.With index of crude protein content of maize stalks, the effects of fermentation time, fermentation temperature, yeast and lactobacillus inoculum on crude protein content were investigated by single factor test. According to the single factor test and optimization on preparing process of fermentation, the optimum fermentation of maize stalks were obtained. In this fermentation conditions, the crude protein content of maize stalks after fermentation was 15. 471% at the conditions of the fermentation time was nine days, the fermentation temperature was 40 ℃ and the inoculation was 1. 5 %.With index of fiber degradation rate of maize stalks, the effects of pH, enzymolysis time, enzymolysis temperature and enzyme concentration on fiber degradation rate were investigated by single factor test. According to the single factor test and optimization on preparing process of enzymolysis, the optimum enzymolysis of maize stalks were obtained. In this enzymolysis conditions, the fiber degradation rate of maize stalks after enzymolysis was 45. 62% at the conditions of the pH was 5. 0, the enzymolysis time was 60 hours, the enzymolysis temperature was 55 ℃ and the enzyme concentration was 50 FPU/g.According to the crude protein content, crude fiber degradation rate, total acid content, total sugar and reducing sugar content of maize stalks, the effects of combination treatment of fermentation and enzymolysis on feedstuff quality of maize stalks were investigated. The results show that when the maize stalks were treated by both fermentation and enzymolysis, the crude protein content was increased by nearly four times than the control group, and 13% higher than a single enzymolysis, 5% higher than a single fermentation. The crude fiber degradation of maize stalks was more significant after combination treatment of fermentation and enzymolysis, which was better than individual fermentation and enzymolysis, and 5.5 times than the control group. After lysozyme together on NDF and ADF degradation rate was 25.57 ± 2.67% and 21.29 ± 1.37%, respectively, enzymatic degradation was better than separate and individual fungi fermentation. After combination treatment of fermentation and enzymolysis, the degradation rate of hemicellulose is 50.61 ± 6.67%, and better than separately. After lysozyme together on cellulose degradation rate is nearly six times higher, reaching 13.54 ± 2.65%, are higher than the degradation rate of double fermentation and digested separately. After combination treatment of fermentation and enzymolysis, the degradation rate of lignin is 49.56 ± 6.65%, significantly higher than the ability to degrade double fermentation and enzyme alone.The total acid content of maize stalks rapidly rising nearly doubled after yeast and lactic acid fermentation, total acid content of maize stalks through enzymatic treatment was also higher than the control group by about 0. 3%, while total acid content after combined treatment was significantly higher than fermentation and enzymolysis separately, and increased one time compared with the control group. The total sugar and reducing sugar content of maize stalks decreased slightly after combination treatment of fermentation and enzymolysis, which may be related to the growth and reproduction of lactic acid bacteria.