Survey On Epidemiology Of Bovine Subclinical Mastitis And Isolation And Identification Of Pathogenic Bacteria In Hulunbeir

The Hulunbuir region is one of China’s largest milk ranch and the rapid development of Hulunbuir’s Dairy Industry is expanding, but due to the low level of local management, the incidence of subclinical mastitis is still high. Subclinical mastitis is One of important factor for restriction develops of dairy industry. Because of the incidence of hidden and difficulty to detect, dairy industry caused huge economic losses.In this research, through investigating subclinical mastitis of the large and medium-scale dairy farm and the state-owned production team in the Hulunbeier four counties, detect the relationship with the quarter, parity and lactation stage and the incidence regularity of recessive dairy cattle mastitis based on the epidemiological investigation method, Somatic cell count method, CMT and mastitis test paper, as a basis of the prevention and control treatment in the future. Sample from the milk of recessive dairy cattle implement some tests,including isolating and identify bacteria,and measuring its sensitivity against medicine. The results showed:1. The statistical analysis of somatic cell data of Hulunbeier area from January 2015 to December. By useing three methods that are Somatic cell count method、CMT and mastitis test paper, in a total of 1533 head, the first positive number is 562, the average positive rate is 36.66% in the region. The higher incidence areas is Chenbaerhu Banner. Overall four pastoral areas in the third and fourth quarter is in a higher prevalence. Analysis of each quarter in the incidence of new diseased cows, we can see that the incidence of bovine subclinical mastitis would vary from month to month changes and the fourth quarter reached a peak incidence; trends of the incidence of subclinical mastitis increased with increasing cow parity continuously; analysis found that the relationship between the incidence of subclinical mastitis cows lactation days is a lower incidence of early lactation cows, on the contrary its high incidence of late lactation.2. Dairy milk sample collection of above four areas, randomly selected from 50 separation of the bacteria identification of recessive mastitis cows, 11 species 119 strains bacteria isolated, identified 29 strains of staphylococcus aureus accounted for 24.4%; Epidermis staphylococcus 14 strains(11.76%), Streptococcus agalactiae 18 strains streptococcus, stop breast 8 strains streptococcus, breast 7 strains streptococcus, fester 2 strains streptococcus, streptococcus a total of 35 strains, accounting for 29.41%; E. coli strains, 21(17.65%), 3 strains klebsiella bacteria, accounting for 2.25%. By staphylococcus aureus, streptococcus pneumoniae and e. coli mastitis caused 83.19% of the total. Results suggest the main pathogenic bacteria in the recessive mastitis dairy cows for staphylococcus aureus, streptococcus and escherichia coli.3. These bacteria detection of Ceftriaxone, Amikacin, Ciprofloxacin, Amoxicillin, Cefotaxime, Enrofloxacin, Ofloxacin, Ampicillin drugs is more sensitive. Oxytetracycline, Tetracycline, Fosfomycin, Neomycin of sensitivity is lower, and for Penicillin G, Erythromycin and other drugs have the very strong resistance.