Identification Of Microsatellite And Analysis Of Genetic Diversity For Farmed Kaluga And Siberian Sturgeon

Sturgeon is a kind of large economic polyploid fish. Due to overharvesting and man-made habitat destruction, sturgeon natural resources in the world were depleting. In this study, for finding the best method of polyploid microsatellite loci reading, three data processing methods including “proportion method”、“filling method”、“1、0 method” were used to compare the different reading result.Thirteen microsatellite loci were amplified among the farmed sterlet(Acipenser ruthenus) population, and the genotypes data were read using these three methods. The results showed that there was no significant difference among three methods of genetic diversity index.Therefore, the method of microsatellite loci reading could be selected according to the experiment methods and sample sources.Twenty microsatellite primers were characterized in Kaluga(Huso dauricus), and 21 microsatellite primers were characterized in Siberian sturgeon(A. baerii). Using 14 of 21 Siberian sturgeon microsatellite primers,7 groups of microsatellite multiple PCR system were established by two primers combination. The population genetic diversity of Kaluga and Siberian sturgeon was analyzed using these microsatellite loci. The results showed that there were 443 and 644 alleles were detected in Kaluga and Siberian sturgeon population respectively, the average effective number of alleles in each population was 9.0759 and 13.4074, and the average PIC value was 0.8595 and 0.8797. Becauseall of PIC value were greater than 0.5, these microsatellite loci were polymorphic. The average observed heterozygosities was0.9534 and 0.9084, average expected heterozygosities was 0.8713 and 0.8892 respectively. The average D values of heterozygote deviation was 0.0942 and 0.0181, it showed that the tested Kaluga and Siberian sturgeon population from sturgeon’s farm had the heterozygote excess phenomenon in a certain degree.Nineteen Kaluga primer pairs could be cross-species amplified in the Siberian sturgeon population. One of these, the primer H53, only was amplified in Kaluga but not be amplified in Siberia sturgeon. In the same way, 14 Siberian sturgeon primer pairs could be cross-species amplified in the Kaluga population. Four primers, Ab8, Ab13, Ab72 and Ab89, only was amplified in Siberia sturgeon but not be amplified inKaluga.The results of genetic diversity analysis showed all detectedlocinumber of alleles were 254 and 345 in Kaluga andSiberian sturgeon populations,the average effective number of alleles was 6.1526 and 11.0464 in this two groups, all of PIC values were greater than 0.5, the average Ho was0.8531 and 0.9459, the average He was 0.8108 and 0.9012. The average D values of heterozygote deviation was 0.0480 and 0.0509. The results showed that there was heterozygosity excess in the farmed Kaluga and Siberian sturgeon populations.The genetic diversity of the population could be increased by introduction of different sources of the parent sturgeons.