In Vitro Toxic Studies Of Cupric Sulfate And Zinc Chloride On Kidney Fin Cell Line In Trachidermus Fasciatus

The 55-65 generation of kidney cell line from roughskin sculpin(Trachidermus fasciatus) were used in this study, the toxic effect of cupric sulfate and zinc chloride.The purpose was to find out the model animals in the coastal estuaries,and to monitor water contamination. The LC50 of cupric sulfate and zinc chloride as estinated by an MTT assay,. was 154.34μmol/L and 308.41μmol/L.SOD activity and GST activity of kidney cell gradually increased with the increasing copper sulfate concentrations over the range of 0 ~ 300μmol/L, reaching a maximum at 300μmol/L, and then gradually decreased.GSH-PX activity reached a maximum at 100μmol/L, and then gradually decreased.With the increasing zinc chloride concentration,SOD、GST and GSH-Px of kidney cell line increased at first and then decreased.The highest activity of SOD and GST occurred when the concentrition was 250μmol/L.GSH-PX activity reached a maximum at 125μmol/L, and then gradually decreased. Micronucleus test indicated that kidney cell produced micronucleus, and the micronucleus rates gradually increased with increasing of copper sulfate and zinc chloride concentrations. Both the micronucleus test and comet assay results showed that there were genotoxic effects on kidney cell line of Trachidermus fasciatus to cupric sulfate and zinc chloride.