Study On The Effects Of Dietary Hesperidin And Chlorogenic Acid On Growth Performance And Intestinal Health In Weaned Piglets

The objective of this study was conducted to investigate the effects of dietary hesperidin(HDN) and hlorogenic acid(CHA) on growth performance, plasma parameters, intestinal mucosa morphology, the expression of intestinal mucosa gene and protein, and microflora of colon in weaned piglets.Forty weaned piglets were randomly divided into four groups(10 piglets per group): control group(CON), CS group(100 mg/kg), HDN group(250 mg/kg), CHA group(250 mg/kg). The experiment lasted 33 d including 5 d adaptation period.The average daily gain and dry matter intake of weaned piglets in HDN and CHA groups were higher than that in CON group(P<0.05). Compared with CON group, feed conversion of piglets in HDN and CHA groups were decreased by 16.3% and 15.9%, respectively(P<0.05). Compared with CS group, decreasedby 8.70% and 8.26%, respectively(P>0.05).The thymu index of piglets in HDN, CHA and CS groups were higher thanthat in CON group(P<0.05). The spleen index of piglets in HDN and CHA groups were higher than that in CON group(P<0.05). The small intestine length index of piglets in HDN and CHA groups were higher than that in CON group(P<0.05). The jejunum length index of piglets in HDN and CHA groups were, compared with CS group, decreased by 10.6% and 12.3% respectively(P>0.05). The ileum length index of piglets in CHA group was higher than that in CS group(P<0.05).The plasma concentration of total antioxidant capacity, total superoxide sismutase, catalase, glutathione reductase, glutathione peroxidase of piglets in HDN and CHA groups were higher than CON group(P<0.05). The plasma concentration of catalase of piglets in HDN and CHA groups were higher than that in CS group(P<0.05). The plasma concentration of total antioxidant capacity and total superoxide dismutase of piglets in HDN and CHA groups were higher than CS group(P>0.05). The plasma concentration of glutathione reductase of piglets in CS group was lower than that inHDN group(P<0.05). Compared with CON group, the plasma concentration of alanine transaminase and aspartate aminotransferase of piglets in the rest three groups were decreased(P<0.05). The plasma concentration of aspartate aminotransferase of piglets in HDN and CHA groups were decreased than that in CS group(P<0.05).The liver concentration of total superoxide dismutase, catalase, glutathione reductase of piglets in CON group was lower than that in another three groups(P<0.05). Compared with CON group, the liver concentration of total antioxidant capacity of piglets in CHA and HDN group were increased by 38.8% and 33.7%, respectively. The liver concentration of total superoxide dismutase of piglets in CS group was lower than that in CHA group(P<0.05). The liver concentration of alanine transaminase and aspartate aminotransferase of piglets in HDN and CHA groups were decreased than that in CON group(P<0.05).The villus height of jejunum of piglets in CON group was lower than that in another groups(P<0.05); CHA group was higher than CS group(P<0.05). The crypt depth of jejunum of piglets in CON group was higher than that in other groups(P<0.05). The villus height / crypt depth of jejunum of piglets in CON group was lower than that in other groups(P<0.05). The villus height of ileum of piglets in was no difference in each group(P>0.05). The crypt depth of ileum of piglets in CHA and HDN groups were lower than that in CON and CS groups(P<0.05). The villus height / crypt depth of ileum of piglets in CON group were lower than that in CHA and HDN groups(P<0.05). The villus height of colon of piglets in CHA group was higher than that in CON group(P<0.05). The crypt depth of colon of piglets in CHA and HDN groups were lower than that in CON group(P<0.05); the villus height / crypt depth of colon of piglets in CHA and HDN groups were higher than that in CON group(P<0.05); HDN group were higher than that in CS group(P<0.05).The expression of β-defensins2 in jejunum of piglets in CON group was lower than that in another three groups(P<0.05); the CS and CHA groups were higher than that in HDN group(P<0.05). There were no difference of the expression of Heat Shock proteinsc 90 and toll-like receptors 4 in jejunum of piglets in every group. The expression of transforming growth factor-β1 in jejunum of piglets in each group were no difference(P>0.05); the expression of Tumor necrosis factor-α in jejunum of piglets in CHA group were higher than CON group(P<0.05); the expression of Interleukin-8 in jejunum of piglets in CHA and HDN groups were no difference compared with CON group(P>0.05); the expression of interleukin-8 in jejunum of piglets in HDN group was significantly higher than that in CS group(P<0.05). Hesperidin and chlorogenic acid can maintain the stability of microbial species in colon in weaned piglets, and protect the intestinal microecological balance.To sum up, supplementing hesperidin and chlorogenic acid can relieve weaning stress, enhance the antioxidant capacity and immune function, and maintain the intestinal microflora balance, promote intestinal development, improve the gowth performance of weaned piglets. Therefore, as a natural additives, hesperidin and chlorogenic acid can be appled to feed production of weaned piglets.