| Dryland winter wheat is one of the most important food crops in Longdong. For a long time, the breeding of winter wheat varieties(lines), the main production as the most preferred breeding goals, but often neglected the selection for other traits, resulting in part of the excellent properties of disappear. With the continuous work of breeding, new varieties(or lines) of parents choose tend to local introduced.cultivation variety(department), lead to bred varieties(or lines) of a single genetic background, its resistance and quality worse and worse. Therefore, in the process of development of winter wheat varieties(lines), how to increase production and ensure the stability of the genetic diversity is particularly important. Longdong region only in understanding the winter wheat genetic abundance core germplasm resources, on the basis of further improved through gene recombination or variation to the quality of the crops and keep its resistance. Therefore, this research in longdong dryland Dong Mai District 56 of winter wheat varieties(or lines) of SSR loci in the genome of allelic variation, natural population genetic structure, important function of HMW GS- gene loci of genetic richness is analyzed; Expectations in examining the genetic heterogeneity between the winter wheat varieties(or lines) on the basis of further insight into the northern dryland Dong Mai District distribution features of germplasm resources, explore more beneficial genes. Research results are as follows:1.we chose 105 pair of primers and 56 winter wheat cultivars from east Gansu Province were evaluated by SSR genetic diversity analyses and population genetic structure analyses in order to comprehend the genetic diversity and population structure of winter wheat in this area. There were 85 pair of primers show polymorphism in this study.2.The results indicated that : there were 243 allelic variation were detected among the 85 pair of primers, the amplitude was in 2 to 6, and there were 2.86 allelic variation per one maker; Allelic variation in group A are among those 80, 92, group B allelic variation group D allelic variation, 71,the average gene diversity was 0.4385; the polymorphism information content(PIC) was cross between 0.0357 to 0.7462, and mean PIC was 0.3824, the average value of validity got from the composition of geneticdiversity of each maker is 93.8%; the amplitude of Shannon index was in 0.0922 to1.5622;3.the amplitude of genetic similarity(GS) was cross between 0.5096 to 0.7606, the mean GS was 0.6414, it could be divided into 5 groups at the 0.6190 of GS; and the group genetic structure analyses showed that the 56 winter wheat cultivars could be divided into 2 subgroup, and there were 29 and 27 winter wheat cultivars in each subgroup respectively.4.There were 10 species of HMW-GS genetic variation in the 56 species of winter wheat, and there were different types of genetic variation. Among them, 3 species in Glu-A1 site(null, 1, 2*), 3 species in Glu-B1 site(7+8, 7+9, 6+8), 4 species in Glu-D1site(2+12, 5+10, 5+12, 2+10). In Glu-1 points, it mainly had null, 7+8 and 2+12 subunit,accounted for 78.01%, 70.42% and 77.75% respectively.5. From the combination type of subunit, there are 15 kinds of subunit combinations in the tested materials. Among them, the subunit null, 7+8, 2+12 had an absolute advantage as its frequency of occurrences reached 35.71%. Followed by null, 7+9, 2+12(19.64%) and 1, 7+8, 2+12(10.71%), the other types of the combination’s frequency of occurrences were below 10%, and the distribution is uniform. High quality subunit combination was 1, 7+8, 5+12(5.36%) and 2*, 7+8, 5+12(1.79%), the frequency of occurrence was generally low. |
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