Study On Microbial Genetic Diversity Inl Arge Leaf Tea Rhizosphere Soil At 11 Mountains From Yunnan Province

Soil is the stronghold of microbe, and the mainly natural habitat.At present, the research on soil microbial community structure of soil habitat has more comprehensive and mature.But for the explore of tea rhizosphere is immature or scarce, especially the large leaf tea tree roots microorganisms of Yunnan. This study combined with high throughput sequencing(Miseq) technology, the Biolog technology, Pure-culture and Biological Information Analysis technology to research the microbial community composition and diversity of the Camellia Sinensis rhizosphere from yunnan mountain of tea,research shows that:1、The use of high-throughput sequencing(Miseq) and Biological Information Analysis technology, to esearch the microbial community composition and diversity that 11 soil samples( Ancient trees:5, Tableland tea garden: 6) from the Camellia Sinensis rhizosphere of yunnan province.We found that: the Camellia Sinensis rhizosphere soil has a unique microbial community composition, main groups of bacteria for Proteobacteria, Acidobacteria, Actinobacteria and Unknown bacteria;Fungal mainly groups are Unknown fungi, Ascomycota and Basidiomycota. the correlation analysis of soil physical and chemical indexes with the microbial community, found the main groups affected by measured index significantly; have the larger proportion of microorganisms undetermined taxa in the two habits, including Unknown bacteria was 7.4%, the proportion of the Unknown fungi is 55%. The experiment and analysis results indicated that the habits has a large number of microbial strains resources unused and the tea rhizosphere microbial community structure is complex.2、Using Biolog technology to explore the growth substrate of 11 soil samples. the result: Carboxylic acid is the optimal effective substrate of JM sample, but amino acids had used effectively by the rest samples. both the Ancient trees habitat and tea garden habitat that related high metabolic activity with substances of amino acids, carboxylic acid, sugar and derivatives, the rest substrate in different types of habitat expressed the diversity of metabolic activity. the results declare that Amino acids, Carboxylic acids, Sugars and so on which are common substance in the environment and living organisms, microbes can choose priority to use.so we mainly should be purely culture with amino acids, Carboxylic acid, Sugar and derivatives substance as key components of medium.3、The results of the pure culture study which are 6 soil samples are follows:Choose 8 substrates to make separation medium(involving 8 class for 4 types),based on 16 s rRNA gene sequence analysis, we get to 2000 strains bacteria, 300 strains fungi, of which 2000 strains bacteria that they belong to 149 similar species, distributed in 59 genera, and from 5 phylum which are Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria. ITS gene Blasted analysis by NCBI blast tool, 300 strains of fungi are included 15 genera and 22 species. the data demonstrated that the culturable microorganisms species diversity of rhizosphere tea is good.