| In order to explore an appropriate forage supply and nutritionally balanced model, and based on the determination of feed intake from grazing, the experiment according to the lactation yields(0.8kg, 1.0kg and 1.2kg respectively) and feeding standard of 40 kg Chinese Merino lactation ewes, three diets(Ⅰ,Ⅱ and Ⅲ) with different nutritional level corresponding to the forage to concentrate ratios of 5:5, 6:4 and 7:3 were confected in this study. These diets were used to conduct grazing supplementary feeding experiment in winter and spring for Gansu Alpine Merino of early lactation, and those lactation ewes of feeding a small amount of concentrates, inferior niblet, large number of alfalfa and oats were designed as control group. And measuring body weight of ewes and lambs, serum biochemical indexes, rumen metabolic parameters, amount of rumen microorganisms, and use 3 nonlinear mathematical model Logistic, Gompertz and Von Bertalanffy, for curve fitting and predictive parsing to growth of the produced lamb by non-experimental ewes. Results showed:1) The end body weight of the 4 groups were higher than that of the original body weight, but have no significantly difference. Prove that the body weight of the 3 supplement groups and the control group in the early lactation did not decline, but also increased. And the lactation ewes average daily gain of groups feed on diet Ⅱ(milk yield 1.0kg)and Ⅲ(milk yield 1.2kg) were higher, respectively as 46.34 g and 49.67 g, significantly higher than of control group(P<0.05). Indicated that grazing Alpine Merino in early lactation feeding diet II(milk yield 1.0 kg), III(milk yield 1. 2kg) can achieve good results on lactating ewes.2) According to the lambs body weight change of the 4 groups, 2-month-old weight of lambs the b group>c group >a group >d group, and the b group was significantly higher than that of control group(P<0.05). 6-month-old weight of lambs the c group>b group >a group >d group, and the c group was significantly higher than that of control group(P<0.05). The 6-month-old weight of lambs(c group) of feed on diet III(C group) was 34.38 kg, higher than 30.94 kg of the control group(Group d) lambs to 3.44 kg. Indicated that grazing Alpine Merino in early lactation feeding on diet III(milk yield 1.2kg) can significantly improve the 6-month-old weight of lambs. According to the average daily gain of lambs, to maximum when 2~3-month-old, and later declined.3) The content of serum GLU by feeding diet Ⅲ was significantly higher than those of another 3 groups(P<0.05). The content of BUN, AST, ALT have no difference among the groups of experiment(P<0.05). The content of serum CHO by feeding diet Ⅱ was significantly higher than those of another 3 groups(P<0.05). The content of serum and End to experiment, the content of serum LDH by feeding diet Ⅱ and Ⅲ were significantly lower than those of another 2 groups(P<0.05).4) The p H of rumen have no difference among the 4 groups(P>0.05). The NH3-N concentration by feeding diet Ⅲ was significantly higher than that of groups feed on diet Ⅱ and control group(P<0.05), and extremely significantly higher than that of group feed on diet Ⅱ(P<0.01). The BUN by feeding diet Ⅰwas highest, and significantly higher than that of group feed on diet Ⅲ(P<0.05). While the Protein N in control group was significantly lower than that of group feed on diet Ⅱ(P<0.05), and extremely significantly lower than that of group feed on diet Ⅰ(P<0.01). The Total N group A > group B > group C > group D, and control group was significantly lower than those of another 3 groups(P<0.05).5) The content of rumen Total VFA by feeding diet Ⅲ was extremely significantly lower than those of another 3 groups(P<0.01). The mole ratios of acetic and Acetic/ propionic by feed on dietⅡ were highest, and extremely significantly higher than that of control group(P<0.01). The mole ratios of propionic by feeding diet Ⅱ was lowest, and was extremely significantly lower than that of group feed on diet Ⅲ and control group(P<0.01).6) The number of protozoa by feeding diet Ⅰand Ⅱ were significantly higher than that of control group(P<0.05).The R.flavefaciens in control group was extremely significantly higher than those of another 3 groups(P<0.01).The R.ablus by feeding diet Ⅰand Ⅱ were extremely significantly lower than that of control group(P<0.01), and was extremely significantly higher than that of group feed on diet Ⅲ(P<0.01). The F.succinogenes by feeding diet Ⅰand Ⅱ were extremely significantly higher than that of control group(P<0.01), while by feeding diet Ⅰwas significantly higher than that of group feed on diet Ⅲ(P<0.05).7) The correlation coefficient R2 were higher than 0.99 of three mathematical model Logistic、Gompertz and Von Bertalanffy. But Gompertz model theoretical inflection point day-old and theoretical average daily gain were higher than those of another 2 model. And more closed to the actual situation of early growth of Gansu Alpine Merino.Overall view, intake diet Ⅰ can significantly raised the BUN, Protein N, Total N concentration, and number of R.flavefaciens, R.ablus, F.succinogenes. Intake diet Ⅱcan significantly affected the average daily gain of lactation ewes and lambs, and the content of serum CHO, TP, ALB, LDH, and can significantly raised the content of rumen Total VFA, mole ratios of acetic, number of protozoa. Intake diet Ⅱcan significantly raised the average daily gain of lactation ewes and lambs, and the content of serum GLU, the content of rumen NH3-N. |
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