Study In Effects Of Light Color On Juvenile And Cloning Of Its Relative Gene In Tomato Clownfish Amphiprion Frenatus

Amphiprion frenatus is belonged to Amphiprioninae, Pomacentridae, Perciformes, the English name is Tomato clownfish. Tomato clownfish is widespread in the sea of western Pacific and eastern Africa, and it is a kind of common marine ornamental fish. As its beautiful appearance and lively habitus that is favored by marine ornamental fish enthusiasts. Now, the demand of marine ornamental fish market is growing, while the wild resources are depleting. A large number of destructive fishing not only led to a reduction of marine ornamental fish, but also take the natural environment into irreparable damage. The study researched the artificial feeding and propagation of tomato clownfish, which is useful for marine ornamental fish aquaculture.1. The artificial propagation of Amphiprion frenatusFirstly, we selected the healthy and lively tomato clownfish fish into artificial pairing, and giving nutrition enhancement prompted its spawning. After 7-10 days, the fertilized eggs hatched into larvae, newly hatched larvae should need nutrition enhancement with rotifers. The attention is that the quality of water during the breeding.2. The effects of different light color on Amphiprion frenatus, juvenileIn the study, we made four treatment groups with different lights which were red, yellow, blue and green, the white light was the control group. Each group was repeated three times, and the experiment lasted for 120 days. The result showed the five treatment groups had no significant difference(P>0.05) in the specific growth rate of juvenile. The red and green treatment groups had significant difference(P<0.05) in the rate of weight gain. The carotene content in red skin of red group was significantly higher than blue, yellow and green(P<0.05), which was also significantly higher in white group than blue group(P<0.05). There was no significantly difference in eye, liver, muscle and white skin with carotene content(P>0.05). The activity of tyrosinase in liver of white group was significantly higher than red group(P<0.05). The activity of tyrosinase in eye, skin and muscle had no significantly difference(P>0.05).3. Cloning and sequence analysis of tyrosinase gene on Amphiprion frenatusThe full-length cDNA of TYR from Tomato clownfish was cloned by RT-PCR and RACE technology. It is composed of 2095 bp, and contained a ORF of 1620 bp, a 5’-UTR of 313 bp, and a 3’-UTR of 162 bp. Phylogenetic analysis showed that the identities of TYR in Tomato clownfish with Channa argus, Takifugu rubripes, Salmon are 85%, 83%, 78%. The phylogenetic tree shows tomato clownfish and gathered in a snakehead, then there is closer relationship with the medaka.Intraspecific genetic relationship between belong to the most recent performance.The study mastered the technology of artificial propagation in tomato clownfish. The light color has a certain impact on tomato clownfish, it shows a increasing skin carotene content in red light environment, and weakened of body tyramine acid activity. It is useful for analysias of tyrosinase gene in tomato clownfish. The findings might provide theoretical guidance for artificial breeding and aquaculture of tomato clownfish.