Mechanism Of Tachyplesinâ…  And Catechin Antitumor Avtivity Of Glioma Cell

The present study is to explore tachyplesinI and catechin which extracted from plants and animals active ingredient on human brain glioma about the mechanism of U251. TachyplesinI is a family of small molecular weight peptides of about 2.3kDa, research confirmed that the blood of horseshoe crabs of marine lower animals with the intervention of tumor cells present in gene expression, regulation of cell proliferation and differentiation of low molecular weight tumor suppressor, but lack of anti-tumor mechanism of a more in-depth study about tachyplesinl. Extraction of catechins in tea polyphenols, inhibit tumor, after the study on the inhibition of glioma has not been reported.Malignant gliomas is a central nervous system tumors, the incidence rate total was reported incidence of domestic intracranial tumors account for about 35.26%-60.96%, average 44.69%. The current treatment of malignant tumors is still a surgery, chemotherapy, radiotherapy combined therapy, glioma is a "leek" type of the tumor, after surgery faster growth and high mortality. Chemotherapy drugs commonly used in clinical cytotoxic effects mainly through the anti-tumor cells, but most of the toxic chemotherapy drugs, patients unbearable. On the other hand, some tumor cells due to chemotherapy or radiation treatment is not sensitive to the cause is not ideal. Therefore, urgent need to develop clinical toxicity, therapeutic effect of a good novel anticancer drugs, to reduce the cancer mortality rate. Carmustine (BCNU) chemotherapy drug of choice as a glioma. fat-soluble, easy to through the blood-brain barrier, inhibiting DNA replication and expression of cell proliferation in the role of both. In this paper, carmustine (BCNU) as control, on the antitumor effect and action tachyplesinl explore local production, the main findings are as follows.The present study is to explore the mechanism of catechin on human brain glioma. Using MTT assay, trypan blue staining cells in rejection. Hoechst33342 staining, cell cycle and other methods confirmed the catechins can inhibit U251 glioma cell growth, so as to further explore the mechanism of catechins to provide an experimental basis for cancer. When the concentration of catechins in the 15.6-250mg/L. the inhibitory effects became clear.Application of tachyplesinI treatment on human glioma cells, and set drug control-chemotherapy drugs carmustine and cell control-normal human glial cells, with cellular biochemistry, cellular and molecular science and other means to deal with cells, research on glial tachyplesinI. The biological effects of U251 tumor cells, and further research tachyplesinl glioma cells in vitro on the mechanism. Established a rubber U251. HEB cell lines in vitro culture system, CCK-8 method and trypan blue cell staining tachyplesinI rejection of human U251 glioma cells inhibited the proliferation, cell morphology was observed by transmission electron microscopy, Hoechst33342/PI fluorescence Detection of tachyplesinl on the U251 cells, DNA agarose gel electrophoresis and the occurrence of apoptosis, flow cytometry cell cycle and apoptosis.With the increasing concentration of catechins, cell swelling became larger, fuzzy outline of the nucleus, mitosis decreased more and more dead cells. Apoptosis rate were dose-dependent manner. The control group did not increase the drug the growth of U251 cells were fibrous, mostly fusiform, the individual was star shape, clear boundaries between cells, shape, full, vigorous growth; 31.25,62.5,125μg/mL catechin treatment U251 After 24h, nuclear condensation, an increasing light blue cells, cells of apoptosis; catechin role U251 cells, the experimental group and control group, with the increasing dose of catechins. S phase fraction gradually down cells were arrested in S phase.When the concentration of 35.33μg/mL tachyplesinl, BCNU, U251 cells in G0/G1 phase DNA content increased (P<0.01), there are very significant in the G0/G1 arrest of produce; tachyplesinl, BCNU concentration of 35.33μg/mL time, HEB cells in S phase DNA content increased (P<0.01), there are very significant in the S phase produce block. 35.33μg/mL the BCNU effect U251, apoptosis rate was 32.9%,35.33μg/mL of tachyplesinl role U251, apoptosis rate was 25.8%.